Summary
The detailed morphology of the nerve fibers and the taste bud cells in developing vallate papillae of the rat tongue was investigated utilizing the immunoperoxidase technique to detect neuron-specific enolase (NSE). For convenience of description, five stages of development were defined: Stage 1, the fifteenth and the sixteenth embryonic day (E15–E16): NSE like immunoreactive (NSE-) nerve fibers, with some random arborization, appeared around the median lingual sulcus at the base of the tongue; Stage 2 (E16–E17): NSE-nerve fibers invading the central core of newly formed vallate papilla and underlying the apical epithelium of the papilla; Stage 3 (E18–E21): round-shaped undifferentiated NSE-taste bud cells appearing in the apical epithelium; Stage 4, the first day of postnatal age (P1): NSE-taste bud cells migrated to the side epithelium, lining the gutter beneath which the nerve plexus formed during E18–E21, and extended cytoplasmic process toward the surface and/or the basal lamina; Stage 5 (P3–P5): NSE-nerve fibers and spindle-shaped NSE-taste bud cells with a typical figure of taste bud cells appeared in newly formed taste buds in the side epithelium, lining the gutter. The sequential topographic development of nerve preceding NSE-taste bud cells in precise morphological locations, suggests that the ingress of precursor NSE-taste bud cells and their subsequent differentiation are contingent upon initial neural derived ontologic signals.
Similar content being viewed by others
Abbreviations
- CRL :
-
crown-rump length
- E1 :
-
the first embryonic day
- NSE :
-
neuron-specific enolase
- NSE :
-
neuron-specific enolase like immunoreactive
- P1 :
-
the first postnatal day
- PAP :
-
peroxidaseanti-peroxidase
- PBS :
-
phosphate buffered saline
References
Farbman AI (1965) Electron microscope study of the developing taste bud in rat fungiform papilla. Dev Biol 11:110–135
Fujita T, Kanno T, Kobayashi S (1988) The paraneuron. Springer-Verlag, Berlin Heidelberg New York London Paris Tokyo, pp 95–102
Hosley MA, Hughes SE, Oakley B (1987) Neural induction of taste buds. J Comp Neurol 260:224–232
Ishikawa Y, Zukeran C, Kuratani S, Tanaka S (1986) A staining procedure for nerve fibers in whole mount preparations of the Medaka and chick embryos. Acta Histochem Cytochem 19:775–783
Jacobson M (1978) Developmental Neurobiology 115–180, Second edition, Plenum Press, London New York
Kuramoto H (1988) An immunohistochemical study of cellular and nervous elements in the taste organ of the bullfrog, Rana catesbeiana. Arch Histol Cytol 51:205–221
Marangos PJ (1987) Neuron specific enolase, a clinically useful marker for neurons and neuroendocrine cells. Ann Rev Neurosci 10:269–295
Torrey TW (1940) The influence of nerve fibers upon taste buds during embryonic development. Proc Natl Acad Sci USA 26:627–634
Toyoshima K, Shimamura A (1988) An immunohistochemical demonstration of neuron-specific enolase in the Merkel cells of the frog taste organ. Arch Histol Cytol 51:295–297
Whitehead MC, Marangos PJ, Connolly SM, Morest DK (1982) Synapse formation is related to the onset of neuron-specific enolase immunoreactivity in the avian auditory and vestibular systems. Dev Neurosci 5:298–307
Yoshie S, Wakasugi C, Teraki Y, Iwanaga T, Fujita T (1988) Immunocytochemical localizations of neuron-specific proteins in the taste bud of the guinea pig. Arch Histol Cytol 51:379–384
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hirata, K., Kanaseki, T. Immunohistochemical studies on neuron-specific enolase in developing rat vallate papillae. Anat Embryol 180, 159–163 (1989). https://doi.org/10.1007/BF00309767
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00309767