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Physical mapping of two temperature-sensitive adenovirus mutants affected in the DNA polymerase and DNA binding protein

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Abstract

We have determined the exact nature of two thermosensitive (ts) adenovirus mutants, H5ts19 and H5ts149, which map to different genes in the E2 transcription unit. The H5ts19 mutation appears to stem from a single base-pair change of A-T to G-C at position 1840 (numbering as in ref. 1), corresponding to codon 154 of the gene coding for DBP. This results in a glutamine-to-arginine change in the amino-terminal domain of the protein. H5ts19 is defective in a late stage of infection, during virus assembly. This phenotype strongly differs from that described for the limited number of known DBP mutants, indicating that DBP is not only functional during DNA replication, but also plays a role in the late phase of the infection cycle.

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References

  1. Kruijer W., van Schaik F.M.A. and Sussenbach J.S., Nucleic Acids Res 9, 4439–4500, 1981.

    Google Scholar 

  2. Sussenbach J.S. in Ginsberg H.S. (ed.) The Adenoviruses. Plenum Press, New York, 1984, pp. 35–124.

    Google Scholar 

  3. Petterson U. in Ginsberg H.S. (ed.) The Adenoviruses. Plenum Press, New York, 1984, pp. 205–270.

    Google Scholar 

  4. Rice S.A., Klessig D.F. and Williams J., Virology 156, 366–376, 1987.

    Google Scholar 

  5. Ginsberg H.S., Ensinger M.J., Kauffman R.S., Mayer A.J. and Lundholm U., Cold Spring Harbor Symp Quant Biol 39, 419–426, 1974.

    Google Scholar 

  6. Lazarides I., Babich A. and Nevins J.R., Virology 165, 438–445, 1988.

    Google Scholar 

  7. Kruijer W., Nicolas J.C., van Schaik F.M.A. and Sussenbach J.S., Virology 124, 425–433, 1983.

    Google Scholar 

  8. Kruijer W., van Schaik F.M.A., Speijer J.G. and Sussenback J.S., Virology 128, 140–153, 1983.

    Google Scholar 

  9. Cleghon V., Voelkerding K., Morin N., Delsert C. and Klessig D., J Virol 63, 2289–2299, 1989.

    Google Scholar 

  10. Ginsberg H.S., Williams J.F., Doerfler W.H. and Shimojo H., J Virol 12, 663–664, 1973.

    Google Scholar 

  11. Williams J.F., Gharpure M., Ustacelebi S. and McDonald S., J Gen Virol 11, 95–101, 1971.

    Google Scholar 

  12. Ustacelebi S. and Williams J.F., Nature 235, 52–53, 1972.

    Google Scholar 

  13. Tarodi B., Metz D.H., Douglas A. and Russell W.C., J Gen Virol 36, 425–436, 1977.

    Google Scholar 

  14. Edvardsson B., Ustacelebi S., Williams J. and Philipson L., J Virol 25, 641–651, 1978.

    Google Scholar 

  15. Tarodi B., Blair G.E., Rekosh M.K. and Russell W.C., J Gen Virol 43, 531–540, 1979.

    Google Scholar 

  16. Young C.S.H., Shenk T. and Ginsberg H.S. in Ginsberg H.S. (ed.) The Adenoviruses. Plenum Press, New York, 1984, pp. 125–172.

    Google Scholar 

  17. KellyJr. T.J. in Ginsberg H.S. (ed.) The Adenoviruses. Plenum Press, New York, 1984, pp 271–308.

    Google Scholar 

  18. Sussenbach J.S. and van der Vliet P.C., Curr Top Microbiol Immunol 109, 53–73, 1983.

    Google Scholar 

  19. Field J., Gronostajski R.M. and Hurwitz J., J Biol Chem 259, 9487–9495, 1984.

    Google Scholar 

  20. Lindenbaum J.O., Field J. and Hurwitz J., J Biol Chem 261, 10218–10227, 1986.

    Google Scholar 

  21. Wilkie N.M., Ustacelebi S. and Williams J.F., Virology 51, 499–503, 1973.

    Google Scholar 

  22. van der Vliet P.C. and Sussenbach J.S., Virology 67, 415–426, 1975.

    Google Scholar 

  23. Ensinger M.J. and Ginsberg H.S., J Virol 10, 328–339, 1972.

    Google Scholar 

  24. Galos R.S., Williams J., Binger M.-H. and Flint S.J., Cell 17, 945–956, 1979.

    Google Scholar 

  25. Gingeras T.R., Sciaky D., Gelinas R.E., Bing-Dong J., Yen C.E., Kelly M.M., Bullock P.A. Parsons B.L., O'Neill K.E. and Roberts R.J., J Biol Chem 257, 13475–13491, 1982.

    Google Scholar 

  26. Aleström P., Akusjärvi G., Pettersson M. and Pettersson U., J Biol Chem 257, 13492–13498, 1982.

    Google Scholar 

  27. Friefeld B.R., Lichy J.H., Hurwitz J. and Horwitz M.S., Proc Natl Acad Sci USA 80, 1589–1593, 1983.

    Google Scholar 

  28. Miller B.W. and Williams J., J Virol 61, 3630–3634, 1987.

    Google Scholar 

  29. Williams J., Galos R.S., Binger M.H. and Flint S.J., Cold Spring Harbor Symp Quant Biol 44, 353–365, 1979.

    Google Scholar 

  30. Williams J.F., Young C.S.H. and Austin P.E., Cold Spring Harbor Symp Quant Biol 39, 427–437, 1974.

    Google Scholar 

  31. Stillman B.W., Tamanoi F. and Mathews M.B., Cell 31, 613–623, 1982.

    Google Scholar 

  32. Pettersson U. and Sambrook J., J Mol Biol 73, 125–130, 1973.

    Google Scholar 

  33. Graham F.L., Smiley J., Russell W.C. and Nairn R., J Gen Virol 36, 59–72, 1977.

    Google Scholar 

  34. Giard D.J., Aaronson S.A., Todaro G.J., Arnstein P., Kersey J.H., Dosik H. and Parks W.P., J Natl Cancer Inst 51, 1417–1423, 1973.

    Google Scholar 

  35. Volkert FC, Munz PL, and Young CSH, Virology, 1989, in press.

  36. Chinnadurai G., Chinnadurai S. and Green M. J Virol 26, 195–199, 1978.

    Google Scholar 

  37. Graham F.L. and van der Eb A.J., Virology 52, 456–467, 1973.

    Google Scholar 

  38. Maxam A.M. and Gilbert W., Proc Natl Acad Sci USA 74, 560–564, 1977.

    Google Scholar 

  39. Sanger F., Nicklen S. and Coulson A.R., Proc Natl Acad Sci USA 74, 5463–5467, 1977.

    Google Scholar 

  40. Dente L., Cesareni G. and Cortese R., Nucleic Acids Res 11, 1645–1655, 1983.

    Google Scholar 

  41. Vos H.L., van der Lee F.M., Reemst A.M.C.B., van Loon A.E. and Sussenbach J.S., Virology 163, 1–10, 1988.

    Google Scholar 

  42. Klessig D.F. and Grodzicker T., Cell 17, 957–966, 1979.

    Google Scholar 

  43. Anderson C.W. and Klessig D.F., J Mol Appl Genet 2, 31–43, 1983.

    Google Scholar 

  44. Johnston J.M., Anderson K.P. and Klessig D.F. J Virol 56, 378–385, 1985.

    Google Scholar 

  45. Anderson K.P. and Klessig D.F. Proc Natl Acad Sci USA 81, 4023–4027, 1984.

    Google Scholar 

  46. Klessig D.F. and Chow L.T. J Mol Biol 139, 221–242, 1980.

    Google Scholar 

  47. Anderson C.W., Hardy M.M., Dunn J.J. and Klessig D.F., J Virol 48, 31–39, 1983.

    Google Scholar 

  48. Brough D.E., Rice S.A., Sell S. and Klessig D.F., J Virol 55, 206–212, 1985.

    Google Scholar 

  49. Vos H.L., Brough D.E., van der Lee F.M., Hoeben R.C., Verheijden G.F.M., Dooijes D., Klessig D.F. and Sussenbach J.S., Virology 172, 634–642, 1989.

    Google Scholar 

  50. Shu L., Hong J.S., Wei Y.-f. and Engler J.A., Gene 46, 187–195, 1986.

    Google Scholar 

  51. Wong S.W., Wahl A.F., Yuan P.-M., Arai N., Pearson B.E., Arai K., Korn D., Hunkapiller M.W. and Wang T.S.-F., EMBO J 7, 37–47, 1988.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory for Physiological Chemistry, State University of Utrecht, Utrecht, The Netherlands

    Dick J. Roovers, Hans L. Vos & John S. Sussenbach

  2. Department of Microbiology, Columbia University, College of Physicians and Surgeons, New York, NY, USA

    C. S. Hamish Young

Authors
  1. Dick J. Roovers
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  2. C. S. Hamish Young
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  3. Hans L. Vos
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  4. John S. Sussenbach
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Correspondence to John S. Sussenbach.

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Roovers, D.J., Young, C.S.H., Vos, H.L. et al. Physical mapping of two temperature-sensitive adenovirus mutants affected in the DNA polymerase and DNA binding protein. Virus Genes 4, 53–61 (1990). https://doi.org/10.1007/BF00308565

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  • DOI: https://doi.org/10.1007/BF00308565

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