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Preparation of histochemical sections for quantitative determination of acid phosphatase activity by means of laser microanalysis

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Summary

Based on former experiments dealing with a quantitative assay of acid phosphatase (acP) activity in histological sections by means of a laser microanalysis. The authors present results of investigations concerning optimal conditions needed for this purpose. The investigations were performed on rat liver sections. The realtion between incubation time and depth of acP reaction appearing in tissue blocks was investigated along with the dependence of photometric readings on thickness of histological sections and on incubation times. From results of experiments it appears that optimal conditions for quantitative determination of acP activity using the proposed laser method are provided by sections 30 to 50 μ in thickness which have been incubated in the substrate containing medium for 45 min. Sections thinner than 30 μ are not recommended for quantitative assay with this method because of low accuracy of readings caused by too low amounts of reaction product present there.

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References

  • Baker, J. R.: The structure and chemical composition of the Golgi element. Quart. J. micr. Sci. 85, 1 (1944).

    Google Scholar 

  • Beatrice, E. S., Harding-Barlow, I., Glick, D.: Electric spark cross excitation in laser microprobe-emission spectroscopy for samples of 10–25 μ diameter. Appl. Spectr. 23, 257–259 (1969).

    Google Scholar 

  • Gomori, G.: Histochemical methods for acid phosphatase. J. Histochem. Cytochem. 4, 453–461 (1956).

    Google Scholar 

  • Kozik, M., Arcimowicz, B., Dembczyński, J.: An attempt of applying, laser rays for the detection and analysis of chemical elements in tissue slices. Acta histochem. (Jena) 37, 203–205 (1970a).

    Google Scholar 

  • —, Warchoł, J., Arcimowicz, B.: The assay of acid phosphatase activity by means of a laser method. Histochemie 24, 245–250 (1970b).

    Google Scholar 

  • Rosan, R. C.: On the preparation of samples for laser microprobe analysis. Appl. Spectr. 19, 97 (1965).

    Google Scholar 

  • —, Brech, F., Glick, D.: Current problems in laser microprobe analysis. Fed. Proc. 24, Suppl. 14, 126 (1965).

    Google Scholar 

  • —, Healy, M. K., McNary, W. F., Jr.: Spectroscopic ultramicroanalysis with a laser. Science 142, 236 (1963).

    Google Scholar 

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Authors and Affiliations

  1. Independent Division of Pathology of the Central Nervous System and Sensory Organs, Medical Academy, Poznan, Poland

    M. Kozik

  2. Department of Histology and Embryology, Medical Academy, Poznań, Poland

    J. Warchoł

  3. Department of Physics, Politechnical High School, Poznań, Poland

    B. Arcimowicz

Authors
  1. M. Kozik
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  2. J. Warchoł
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  3. B. Arcimowicz
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Kozik, M., Warchoł, J. & Arcimowicz, B. Preparation of histochemical sections for quantitative determination of acid phosphatase activity by means of laser microanalysis. Histochemie 26, 74–79 (1971). https://doi.org/10.1007/BF00307786

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  • DOI: https://doi.org/10.1007/BF00307786

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