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Some observations on a histochemically demonstrable leucyl-naphtylamidase activity (cathepsin B?) in chicken liver

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Summary

A histochemically demonstrable leucyl-naphtylamide-splitting enzyme activity in the lipid droplets of normal chicken liver cells is described. It resists fixation with formaldehyde but not with glutaraldehyde. Its demonstration is sensitive to the acetate concentration in the reaction mixture but not to metal ions or -SH groups. It is optimally demonstrated at pH 5.5 and differs thus significantly from the aminopeptidases. It is suggested that this activity may be identical with the cathepsin B found by others in the lysosomes of dying cells.

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Authors and Affiliations

  1. Department of Human Anatomy, University of Uppsala, Uppsala, Sweden

    Björn Sandström

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  1. Björn Sandström
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This study was supported in part by a grant from the Swedish Medical Research Council.

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Sandström, B. Some observations on a histochemically demonstrable leucyl-naphtylamidase activity (cathepsin B?) in chicken liver. Histochemie 26, 35–39 (1971). https://doi.org/10.1007/BF00307781

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  • DOI: https://doi.org/10.1007/BF00307781

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