Skip to main content
Log in

Absorption of horse-radish peroxidase by the mucosal cells of the duodenum of mouse

II. The newborn mouse

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

Horse-radish peroxidase was injected intraperitonealy to fasted and fed newborns. The protein was rapidly absorbed by the fasting animals and more slowly by the fed newborns. The tracer was progressively segregated into the supranuclear cluster of dense tubules and dense bodies. The cellular sap of several cells was deeply stained after the intraperitoneal injection. Control experiments showed that peroxidase has a high affinity for the matrix of the dense bodies in the first days of neonatal life.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Bibliography

  • Becker, N. H., Novikoff, A. B., Zimmerman, H. M.: Fine structure observations of the uptake of intravenously injected peroxidase by the rat choroid plexus. J. Histochem. Cytochem. 15, 160–165 (1967).

    Google Scholar 

  • Behnke, O.: Demonstration of endogenous peroxidase activity in the electron microscope. J. Histochem. Cytochem. 17, 62–64 (1969).

    Google Scholar 

  • Clementi, F., Palade, G. E.: Intestinal capillaries. 1. Permeability to peroxidase and ferritin. J. Cell Biol. 41, 33–58 (1969).

    Google Scholar 

  • Cornell, R., Padykula, H. A.: A cytological study of intestinal absorption in the suckling rat. Amer. J. Anat. 125, 291–316 (1969).

    Google Scholar 

  • Cotran, R. S., Karnovsky, M. J.: Ultrastructural studies on the permeability of the mesothelium to horseradish peroxidase. J. Cell Biol. 37, 123–137 (1968).

    Google Scholar 

  • Essner, E.: Localization of peroxidase activity in microbodies of fetal mouse liver. J. Histochem. Cytochem. 17, 454–466 (1966).

    Google Scholar 

  • —: Observations on hepatic and renal peroxisomes (microbodies) in the developing chick. J. Cytochem. Histochem. 18, 80–92 (1970).

    Google Scholar 

  • Fahimi, D. H.: Cytochemical localization of peroxidase activity in rat hepatic microbodies (peroxisomes). J. Histochem. Cytochem. 16, 547–550 (1968).

    Google Scholar 

  • —: Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (peroxisomes). J. Cell Biol. 43, 275–288 (1969).

    Google Scholar 

  • Graham, R. C., Karnovsky, M. J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966).

    Google Scholar 

  • Graney, D. O.: The uptake of ferritin by ileal absorptive cells in suckling rats. An electron microscope study. Amer. J. Anat. 123, 227–254 (1968).

    Google Scholar 

  • Hampton, J. C., Rosario, B.: The distribution of exogenous peroxidase in irradiated mouse intestine. Radiat. Res. 34, 209–227 (1968).

    Google Scholar 

  • Holtzman, E., Dominitz, R.: Cytochemical studies of lysosomes, Golgi apparatus and endoplasmic reticulum in secretion and protein uptake by adrenal medulla cells of the rat. J. Histochem. Cytochem. 16, 320–336 (1968).

    Google Scholar 

  • Hugon, J. S.: Ultrastructural differentiation and enzymatic localization of phosphatases in the developing duodenal epithelium of the mouse. 11. The newborn mouse. Histochemie 22, 109–124 (1970)

    Google Scholar 

  • —, Borgers, M.: Absorption of horseradish peroxidase by the mucosal cells of the duodenum of mouse. 1. The fasting animal. J. Histochem. Cytochem. 16, 229–236 (1968).

    Google Scholar 

  • Kraehenbuhl, J. P., Campiche, M. A.: Early stages of intestinal absorption of specific antibodies in the newborn. J. Cell Biol. 42, 345–365 (1969).

    Google Scholar 

  • Like, A. A.: The uptake of exogenous peroxidase by the beta cells of the islets of Langerhans. Amer. J. Path. 59, 225–245 (1970).

    Google Scholar 

  • Matter, A., Orci, L., Rouiller, C.: A study on the permeability barriers between Disse's space and the bile canaliculus. J. Ultrastruct. Res. 11, October 1969.

  • Miller, A. T., Hale, D. M., Alexander, K. D.: Histochemical studies on the uptake of horseradish peroxidase by rat kidney slices. J. Cell Biol. 27, 305–312 (1965).

    Google Scholar 

  • Papadimitriou, J. M., Walters, M. N.-I.: Fluid flow in the liver demonstrated with horse radish peroxidase. Amer. J. Anat. 123, 475–488 (1968).

    Google Scholar 

  • Poux, N.: Localisation d'activités enzymatiques dans les cellules du méristème radiculaire de Cucumis Sativus L. 11. Activité peroxydasique. J. Microscopie 8, 855–866 (1969).

    Google Scholar 

  • Rodewald, R.: Selective antibody transport in the proximal small intestine of the neonatal rat. J. Cell Biol. 45, 635–640 (1970).

    Google Scholar 

  • Schneeberger-Keeley, E. E., Karnovsky, M. J.: The ultrastructural basis of alveolar-capillary membrane permeability to peroxidase used as a tracer. J. Cell Biol. 37, 781–793 (1968).

    Google Scholar 

  • Straus, W.: Use of horseradish peroxidase as a marker protein for studies of phagolysomes, permeability and immunology. Meth. Achievm. exp. Path. 4, 54–91 (1969).

    Google Scholar 

  • Strum, J. M., Karnovsky, M. J.: Ultrastructural localization of peroxidase in submaxillary acinar cells. J. Ultrastruct. Res. 31, 323–336 (1970a).

    Google Scholar 

  • —: Cytochemical localization of endogenous peroxidase in thyroid follicular cells. J. Cell Biol. 44, 655–666 (1970b).

    Google Scholar 

  • Venkatachalam, M. A., Karnovsky, M. J., Cotran, R. S.: Glomerular permeability. Ultrastructural studies in experimental nephrosis using horseradish peroxidase as a tracer. J. exp. Med. 130, 381–399 (1969).

    Google Scholar 

  • —, Soltani, M. H., Fahimi, H. D.: Fine structural localization of peroxidase activity in the epithelium of large intestine of rat. J. Cell Biol. 46, 168–173 (1970).

    Google Scholar 

  • Wissig, S. L., Graney, D.O.: Membrane modifications in the apical endocytic complex of ileal epithelial cells. J. Cell Biol. 39, 564–579 (1968).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This work was supported by grants no. MA-3994 Medical Research Council of Canada, and by no. 9340-06 National Defense Board.

The author is greatly indebted to Mr. Michel Couture for his skillful technical aid.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hugon, J.S. Absorption of horse-radish peroxidase by the mucosal cells of the duodenum of mouse. Histochemie 26, 19–27 (1971). https://doi.org/10.1007/BF00307779

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00307779

Keywords

Navigation