Summary
The connective tissue of the mucosa of the respiratory tract, of the gastric mucosa and of the mucosa of the tongue was investigated in mice. The tissue was fixed in glutaraldehyde and incubated in an alkaline DAB-medium to demonstrate the peroxidatic activity of catalase. In fibroblasts and fibrocytes, as well as in lymphoid cells, membrane bounded particles from 0.10 to 0.25 μm in diameter were found, whose matrices were intensely stained by the histochemical reaction. The reaction is inhibited by the addition of 2×10−2 M 3-amino-1,2,4-triazole. In connective tissue cells of specimens, which were not reacted to demonstrate catalase activity, these organelles show a granular matrix of moderate electron density. They lack a crystalline core. The possibility that these catalase-positive particles (CPs) represent peroxisomes is discussed.
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This investigation was kindly supported by a grant of “Hochschuljubiläumsstiftung der Stadt Wien”.
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Böck, P. Cytochemical demonstration of catalasepositive particles (peroxisomes?) in fibroblasts. Z.Zellforsch 144, 539–547 (1973). https://doi.org/10.1007/BF00307379
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DOI: https://doi.org/10.1007/BF00307379