Summary
Mice were immunized by repeated intraperitoneal injections of pig granulocytes, at 48 hour intervals. Animals were sacrificed 18 hours after the last injection. Stimulated and non-stimulated omenta were cultivated for various times up to 18 days. In the highly developed population of cells migrating from the stimulated explants, histiocytes with a large, extended surface appeared by the 6th day of growth. When cultures were incubated in vitro with the antigen, the histiocytes were capable of fixing granulocytes on their surface. This phenomenon was especially displayed by the large histiocytes. In control cultures, resulting from non-stimulated omenta, adherent granulocytes were only occasionally observed and no large histiocytes appeared. — The significance of these figures of adherence with respect to the immune treatment is discussed.
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Loni, M.C. Adhérence de granulocytes, utilisés in vivo lors d'une stimulation repetée, sur des cellules histiocytaires issues de l'épiploon de souris cultivé in vitro . Z.Zellforsch 141, 505–515 (1973). https://doi.org/10.1007/BF00307121
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DOI: https://doi.org/10.1007/BF00307121