Summary
Lysosomal aryl sulphatase has been localised in the lung at the electron microscopic level using a nitrocatechol sulphate barium chloride medium. Variations in fixative concentration and incubation time were found to be important in minimising lysosomal leakage. The distribution of aryl sulphatase in the lung corresponded closely to that of acid phosphatase. Large amounts were found in alveolar macrophages and small quantities in the type II alveolar epithelial cell. In the latter cell the enzyme was found in the lamellar vacuoles thought to represent the site of surfactant production. The significance of this in regard to the function of these organelles is discussed.
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Corrin, B., A. E. Clark, and H. Spencer: Ultrastructural localisation of acid phosphatase in the rat lung. J. Anat. (Lond.) (in the press).
Goldfischer, S., Y. Kikkawa, and L. Hoffman: The demonstration of acid hydrolase activities in the inclusion bodies of type II alveolar cells and other lysosomes in the rabbit lung. J. Histochem. Cytochem. 16, 102–109 (1968).
Hatasa, K., and T. Nakamura: Electron microscopic observations of lung alveolar epithelial cells of normal young mice, with special reference to the formation and secretion of osmiophilic lamellar bodies. Z. Zellforsch. 68, 266–277 (1965).
Hugon, J., and M. Borgers: Fine structural localisation of lysosomal enzymes in the absorbing cells of the duodenal mucosa of the mouse. J. Cell Biol. 33, 212–218 (1967).
Klaus, M., O. K. Reiss, W. H. Tooley, C. Piel, and J. A. Clements: Alveolar epithelial cell mitochrondria as source of surface-active lung lining. Science 137, 750–751 (1962).
Niden, A. H.: Bronchiolar and large alveolar cells in pulmonary phospholipid metabolism. Science 158, 1323–1324 (1967).
Rowden, G.: Lysosomal aryl sulphatase in mouse kidney and liver. Nature (Lond.) 215, 1283–1284 (1967).
Roy, A. B.: The synthesis and hydrolysis of sulphate esters. Advanc. Enzymol. 22, 205–235 (1960).
Semadeni, E. G.: Enzymatische Charakterisierung der Lysosomenäquivalente (Sphärosomen) von Maiskeimlingen. Planta (Berl.) 72, 91–118 (1967).
Sorokin, S.: A morphologic and cytochemical study on the great alveolar cell. J. Histochem. Cytochem. 14, 884–897 (1966).
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Corrin, B., Clark, A.E. Lysosomal aryl sulphatase in pulmonary alveolar cells. Histochemie 15, 95–98 (1968). https://doi.org/10.1007/BF00306361
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DOI: https://doi.org/10.1007/BF00306361