Skip to main content
SpringerLink
Log in

H and B human blood-group antigen expression in cochlear hair cells is modulated by thyroxine

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Abstract

The presence of human blood-group antigens in developing and adult hypothyroid rat cochleas was analyzed using antibodies directed against antigens H and B. During postnatal development, hypothyroid rat cochleas exhibited a highly selective expression of both B and H antigens, mainly at the hair cell level. Labeling for antigen B was found throughout the hair cells, whereas the antibody directed against antigen H selectively labeled the apical part of these cells. These immunostaining patterns were similar to those found in normal (euthyroid) rat cochleas, but antigenic expression periods were clearly prolonged. Thus, whereas in normal rat cochleas, the B and H antigenic expression disappears from postnatal day (PD) 9 on, in cochleas of hypothyroid rats the reactivity was intense until PD15; it decreased from this developmental stage, and was negative or only faintly positive at PD30. Therefore, in congenital hypothyroidism, hair cell immunoreactivity is present at developmental stages that are negative in normal rat cochleas. These results suggest that human blood-group antigen expression on the developing cochlear hair cells of rats is modulated by thyroxine and that thyroxine is necessary for the temporal expression pattern and secretion of normal glycoproteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Astic L, Le Pendu J, Mollicone R, Saucier D, Oriol R (1989) Cellular expression of H and B antigens in the rat olfactory system during development. J Comp Neurol 289:386–394

    Google Scholar 

  • Chester MA, Johnson U, Lundblad A, Löw B, Messeter L, Samuelsson B (1990) Proceedings of the 2nd International Workshop on Monoclonal Antibodies against Human Red Cells and Related Antigens. April 1–4 1990, Lund, Sweden, pp 208–209

  • Chou DK, Dodd J, Jessell TM, Costello CE, Jungalwala FB (1989) Identification of α-galactose (α-fucose)-asialo GM1 glycolipid expressed by subsets of rat dorsal root ganglion neurons. J Biol Chem 264:3409–3415

    Google Scholar 

  • Christie GA (1964) Developmental stages in somite and post-somite rat embryos, based on external appearance, and including some features of the macroscopic development of the oral cavity. J Morphol 11:263–286

    Google Scholar 

  • Dabelsteen E, Vedtofte P, Hakomori S, Young WW (1982) Carbohydrate chains specific for blood group antigens in differentiation of human oral epithelium. J Invest Dermatol 79:3–7

    Google Scholar 

  • Dabelsteen E, Buschard K, Hakomori S, Young WW (1984) Pattern of distribution of blood group antigen on human epidermal cells during maturation. J Invest Dermatol 82:13–17

    Google Scholar 

  • Damjanov I (1987) Biology of disease. Lectin cytochemistry and hystochemistry. Lab Invest 57:5–20

    Google Scholar 

  • De Grauw T, Liwniez B (1986) Lectins are markers of neuronal migration and differentiation in rat brain. Dev Neurosci 8:236–242

    Google Scholar 

  • Debruyne F, Vanderschueren-Lodeweyckx M, Bastijns P (1983) Hearing in congenital hypothyroidism. Audiology 22:404–409

    Google Scholar 

  • Deol MS (1973) An experimental approach to the understanding and treatment of hereditary syndromes with congenital deafness and hypothyroidism. J Med Gene 10:235–242

    Google Scholar 

  • Deol MS (1976) The role of thyroxine in the differetiation of the organ of Corti. Acta Otolaryngol (Stockh) 81:429–435

    Google Scholar 

  • Dodd J, Jessell TM (1985) Lactoseries carbohydrates specify subsets of dorsal root ganglion neurons projecting to the superficial dorsal horn of rat spinal cord. J Neurosci 5:3278–3294

    Google Scholar 

  • Edelman GM (1983) Cell adhesion molecules. Science 219:450–457

    Google Scholar 

  • Edelman GM (1984) Cell adhesion and morphogenesis: the regulator hypothesis. Proc Natl Acad Sci USA 81:1460–1464

    Google Scholar 

  • Feizi T (1985) Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are oncodevelopmental antigens. Nature 314:53–57

    Google Scholar 

  • Feizi T, Childs RA (1987) Carbohydrates as antigenic determinants of glycoproteins. Biochem J 245:1–11

    Google Scholar 

  • Gabrion J, Legrand CH, Mercier B, Harricane MC, Uziel A (1984) Microtubules in the cochlea of the hypothyroid developing rats. Hear Res 13:203–214

    Google Scholar 

  • Gil-Loyzaga P, Pujol R, Mollicone R, Dalix AM, Oriol R (1989) Appearance of B and H blood group antigens in the developing cochlear hair cells. Cell Tissue Res 257:17–21

    Google Scholar 

  • Gil-Loyzaga P, Menoyo A, Poch J, Merchán-Pérez A (1990) Effects of perinatal hypothyroidism in the carbohydrate composition of cochlear tectorial membrane. Hear Res 45:151–156

    Google Scholar 

  • Gil-Loyzaga P, Remezal M, Oriol R (1992) Neuronal influence on B and H human blood-group antigen expression in rat cochlear cultures. Cell Tissue Res 269:13–20

    Google Scholar 

  • Hakomori S (1981) Blood group A, B, H and ii antigens of human erythrocytes: chemistry, polymorphism, and their development changes. Semin Hematol 18:39–62

    Google Scholar 

  • Haubrich J (1975) Tierexperimentelle histologisch-histochemische Untersuchungen zur Formalgenese der hypothyreotisch bedingten Schwerhörigkeit. Acta Otolaryngol (Stockh) [Suppl] 332:1–56

    Google Scholar 

  • MacKenzie IC, Dabelsteen E (1987) Connective tissue influences on the expression of epithelial cell surface antigens. Cell Tissue Res 248:137–141

    Google Scholar 

  • Meyerhoff WL (1976) The thyroid and audition. Laryngoscope 86:483–489

    Google Scholar 

  • Mollicone R, Trojan J, Oriol R (1985) Appearance of H and B antigens in primary sensory cells of the rat olfactory apparatus and inner ear. Dev Brain Res 17:275–279

    Google Scholar 

  • Mollicone R, Davies DR, Evans B, Dalix AM, Oriol R (1986) Cellular expression and genetic control of ABH antigens in primary sensory neurons of marmoset, baboon and man. J Neuroimmunol 10:255–269

    Google Scholar 

  • Morreale de Escobar G, Obregon MJ, Ruiz de Oña C, Escobar de Rey F (1988) Transfer of thyroxine from the mother to the rat fetus near term: effects on brain 3,5,3′-triiodothyronine deficiency. Endocrinology 122:1521–1531

    Google Scholar 

  • Oriol R (1990) Genetic control of the fucosylation of ABH precursor chains. Evidence for new epistatic interactions in different cells and tissues. J Immunogenet 17:235–245

    Google Scholar 

  • Oriol R (1993) ABO, Hh, Lewis and Secretion. Serology genetics and tissue distribution. In: Cartron JP, Rouger P (eds) Molecular basis of major blood group antigens. Blood cell biochemistry, vol 6. Plenum Press, New York (in press)

    Google Scholar 

  • Oriol R, Mancilla-Jiménez R (1983) Fluorescent staining nuclei and amyloid substance. Two useful properties of p-phenylenediamine. J Immunol Methods 62:185–192

    Google Scholar 

  • Oriol R, Cooper JE, Davies DR, Keeling PWN (1984) ABH antigens in vascular endothelium and some epithelial tissues of baboons. Lab Invest 50:514–518

    Google Scholar 

  • Oriol R, Le Pendu J, Mollicone R (1986) Genetics of ABO, Lewis, X and related antigens. Vox Sang 51:161–171

    Google Scholar 

  • Pfenninger KH, Maylie-Pfenninger MF, Friedmann LB, Simkowitz P (1984) Lectin labelling of sprouting neurons III: type specific glycoconjugates on growth cones of different origin. Dev Biol 106:97–108

    Google Scholar 

  • Prieto JJ, Rueda J, Sala ML, Merchan JA (1990a) Lectin staining of saccharides in the normal and hypothyroid developing organ of Corti. Dev Brain Res 52:141–149

    Google Scholar 

  • Prieto JJ, Rueda J, Merchan JA (1990b) The effect of hypothyroidism on the development of the glycogen content of organ of Corti's hair cells. Dev Brain Res 51:138–141

    Google Scholar 

  • Reibel J, Dabelsteen E, Hakomori S, Young WW, Mackenzie IC (1984) The distribution of blood group antigens in rodent epithelia. Cell Tissue Res 237:111–116

    Google Scholar 

  • Remezal M, Gil-Loyzaga P (1993) Incorporation of D3H glucosamine to the adult and developing cochlear tectorial membrane of normal and hypothyroid rats. Hear Res 66:23–30

    Google Scholar 

  • Remezal M, Gil-Loyzaga P, Mollicone R, Oriol R (1993a) Immunocytochemical approach to the structure of human blood group B and H glycoconjugate antigens in the immature rat cochlea. Cell Tissue Res 274:21–26

    Google Scholar 

  • Remezal M, Gil-Loyzaga P, Oriol R (1993b) Ultrastructural localization of H human-blood-group immunoreactivity in three days old rat cochlear hair cells. ORL J Otorhinolaryngol Relat Spec 55:205–207

    Google Scholar 

  • Remezal M, Gil-Loyzaga P, Mollicone R, Joubert-Caron R, Oriol R (1993c) Histochemical localization of a β-galactoside-binding lectin and its binding-sites in developing and adult rat cochlea. Dev Brain Res 73:1–6

    Google Scholar 

  • Roth J, Taatjes DJ, Bitter-Suermann D, Finne J (1987) Polysialic acid units are spatially and temporally expressed in developing postnatal rat kidney. Proc Natl Acad Sci USA 84:1969–1973

    Google Scholar 

  • Rutishauser U (1984) Developmental biology of a cell adhesion molecule. Nature 310:594–597

    Google Scholar 

  • Steel K (1980) The proteins of normal and abnormal tectorial membrane. Acta Otolaryngol (Stockh) 89:27–32

    Google Scholar 

  • Thorpe SJ, Bellairs R, Feizi T (1988) Developmental patterning of carbohydrate antigens during early embryogenesis of the chick: expression of antigens of the poly-N-acetyllactosamine series. Development 102:193–210

    Google Scholar 

  • Trotter WR (1960) The association of deafness with thyroid dysfunction. Br Med Bull 16:92–98

    Google Scholar 

  • Uziel A, Rabie A, Marot M (1980) The effect of hypothyroidism on the onset of cochlear potentials in developing rats. Brain Res 182:172–175

    Google Scholar 

  • Uziel A, Gabrion J, Ohresser J, Legrand C (1981) Effects of hypothyroidism on the structural development of the organ of Corti in the rat. Acta Otolaryngol (Stockh) 92:469–480

    Google Scholar 

  • Uziel A, Legrand C, Ohresser M, Marot M (1983) Maturational and degenerative processes in the organ of Corti after neonatal hypothyroidism. Hear Res 11:203–218

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratorio de Neurobiología Sensorial y Audiologá, Dept. Ciencias Morfologicas, Universidad Complutense, Apartado de Correos 60075, E-28080, Madrid, Spain

    Pablo Gil-Loyzaga, Manuel Remezal & Angeles Ibáñez

  2. INSERM U.178, 16 Avenue Paul Vaillant-Couturier, F-94807, Villejuif, France

    Rosella Mollicone & Rafael Oriol

Authors
  1. Pablo Gil-Loyzaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manuel Remezal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rosella Mollicone
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Angeles Ibáñez
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rafael Oriol
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gil-Loyzaga, P., Remezal, M., Mollicone, R. et al. H and B human blood-group antigen expression in cochlear hair cells is modulated by thyroxine. Cell Tissue Res 276, 239–243 (1994). https://doi.org/10.1007/BF00306109

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00306109

Key words

Search

Navigation