Summary
Histochemical study in epiphyseal cartilages and periodontal tissues of the rat subjected to cortisone and PTH administration was examined enzyme histochemically in the decalcified frozen sections with neutral EDTA.
In cortisone and PTH-treated rats, altered proliferating cartilage cells in the epiphysis revealed a weak, and decreasing activities of oxidative enzymes with the comparison to those of the control, however, they showed similar activity for alkaline and acid phosphatases as normal cells.
Epiphysis and alveolar bones in large dose of cortisone administered rats exhibited an absence or markedly decreasing number of osteoclasts, but on the contrary, those of PTH treated rats led to an increased number of the cells. Osteoclasts in hormone treated conditions were characterized by the existence of the strong succinate dehydrogenase activity as well as in normal status.
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References
Anneroth, G., Bloom, G.: Structural changes in the incisors of cortisone-treated rats. J. dent. Res. 45, 229–235 (1966).
Balogh, K., Jr.: Histochemical study of oxidative systems in teeth and periodontal tissues. J. dent. Res. 42, 1457–1466 (1963).
—, Dudley, H. R., Cohen, R. B.: Oxidative enzymes activity in skeletal cartilage and bone. Lab. Invest. 10, 839–845 (1962).
Bernick, S., Ershoff, B. H.: Histochemical study of bone in cortisone-treated rats. Endocrinology 72, 231–237 (1963).
—, Zipkin, I.: Histochemical study of bone in hydrocortisone-treated and fluoride-treated rats. J. dent. Res. 46, 1404–1411 (1967).
D'Arcy, P. F., Howard, E. M.: The effect of prolonged administration of cortisone acetate on the weight of the various organs of the rats. Mem. Soc. Endocr. 10, 46–48 (1961).
Deguchi, T., Mori, M.: Histochemical observation on oxidative enzymes in periodontal tissues during experimental tooth movement in the rat. Arch. oral. Biol. 13, 49–58 (1968).
Follis, R. H.: Effect of cortisone on growing bones in the rats. Proc. Soc. exp. Biol. 76, 722–724 (1951).
Fullmer, H. M.: Dehydrogenases in developing bone in the rat. J. Histochem. Cytochem. 12, 210–214 (1964).
—, Link, C., Jr: A demineralization procedure for enzymatic histochemical use: A quantitative succinic dehydrogenase assay. Stain Technol. 39, 387–396 (1964).
Garren, L., Greep, R. O.: Effect of adrenal cortical hormones on eruption rate of incisor teeth in the rat. J. Endocr. 66, 625–628 (1960).
Gibson, W. A., Fullmer, H. M.: Histochemistry of the periodontal ligament. Periodontics 4, 63–70 (1966).
Goldsmith, E. D.: Effect of long-therm cortisone treatment on the supporting dental structures of the rat. J. Dent. Res. 32, 699 (1953).
—, Ross, T. A.: Histochemical and histological study of the effects of cortisone on the lower incisors of fetal and postnatal rats. Acta endocr. (Kbh.) 22, 23–25 (1956).
Hekkelmann, J. W.: The parathyroids, Chikago: Chicago Univ. Press p. 211. 1965.
Meyer, W. L., Kunin, A. S.: Decreased glycolytic enzyme activity in epiphyseal cartilage of cortisone-treated rats. Arch. Biochem. 129, 431–437 (1969).
Mori, M., Ito, M., Fukui, S.: Decalcification for histochemical demonstration of hydrolytic and oxidative enzymes. Histochemie 5, 185–195 (1965).
Sissons, H. A.: The influence of cortisone on the structure and growth of bone. J. Anat. (Lond.) 89, 66–78 (1954).
Takada, K.: Enzyme histochemistry in bone tissue. I. Histochemical detection of oxidative enzymes in developing knee joints of rats. Acta histochem. (Jena) 23, 40–52 (1966).
Takimoto, K., Deguchi, T., Mori, M.: Histochemical detection of acid and alkaline phosphatases in periodontal tissues after experimental tooth movement. J. dent. Res. 47, 340 (1968).
Talmage, R. V.: The effect of actinomycin D (AMD) on parathyroid function in the rat. Proc. Ann. Meet. Endocrine. Soc. Abstract 21 (1965).
Todo, H., Mori, M.: Histochemical observations on hydrolytic and oxidative enzymes in tooth supporting tissues in mature rodents. Arch. oral. Biol. 11, 921–930 (1966).
Toft, R. J., Talmage, R. V.: Quantitative relationship of osteoclasts to parathyroid function. Proc. Soc. exp. Biol. (N. Y.) 103, 611–613 (1960).
Weidmann, S. M.: Calcification of skeletal tissues. In: Inter. Review of Connective Tissues Research (D. A. Hall), vol. 1, p. 339–377. New York and London: Academic Press 1963.
Weinmann, J. P., Schour, I.: The effect of parathyroid hormone on the alveolar bone and teeth of the normal and rachitic rat. Amer. J. Path. 21, 857–875 (1945).
—, Sicher, H.: Bone and bones, 2. ed. 25. St. Louis: C. V. Mosby Co. 1955.
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Deguchi, T., Mori, M. Histochemical observations on bone and periodontal tissues in the rat administered with cortisone and parathyroid hormones. Histochemie 20, 234–243 (1969). https://doi.org/10.1007/BF00306011
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DOI: https://doi.org/10.1007/BF00306011