Summary
By starch gel electrophoresis three mobility variants of a cathodic moving doublet of bands, encoded by the structural gene prxC, were detected in all organs of flowering petunias. In root tissue two of the variants showed a lower electrophoretic mobility than in other organs. During development of flower buds the PRXc enzymes showed an increase in mobility. The gene prxC was located on chromosome IV by showing linkage to the genes An3 and Dw1, by trisomic segregation, and by the construction of triply heterozygous trisomics IV. The gene order on chromosome IV is B1-An3/Dw1-prxC. It was concluded that the temporal programming difference in the expression of the alleles prxC2 and prxC3 is caused by internal site mutation. Analysis of progeny obtained by crossing of lines to the trisomic IV with genotype prxC1/C1/C2 showed differential expression of the two prxC1 alleles of the trisomic IV.
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Literature
Benito, C.; Pérez de la Vega, M. (1979): The chromosomal location of peroxidase isozymes of the wheat kernel. Theor. Appl. Genet. 55, 73–76
Benito, C.; Pérez de la Vega, M.; Salinas, J. (1980): The inheritance of wheat kernel peroxidases. J. Hered. 71, 416–418
Berg, B.M. van den; Wijsman, H.J.W. (1981): Genetics of the peroxidase isoenzymes in Petunia. Part 1: Organ specificity and general genetic aspects of the peroxidase isoenzymes. Theor. Appl. Genet. 60, 71–76
Berg, B. M. van den; Wijsman, H.J.W. (1982a): Genetics of the peroxidase isoenzymes in Petunia. Part 2: Location and developmental expression of the structural gene prxB. Theor. Appl. Genet. 61, 297–303
Berg, B.M. van den; Wijsman, H.J.W. (1982b): Genetics of the peroxidase isoenzymes in Petunia. Part 3: Location and developmental expression of the structural gene prxA. Theor. Appl. Genet. 63, 33–38
Denna, D.W.; Alexander, M.B. (1975): The isoperoxidases of Curcubita pepo L. In: Isozymes II (ed. Markert, C.L.), pp. 851–864. New York: Acad. Press
Endo, T. (1981b): Differential regulation of peroxidase isozymes coded by Px-1 locus in rice. Jpn. J. Genet. 56, 175–183
Felder, M.R. (1976): Genetic control of four cathodal peroxidase isoenzymes in barley. J. Hered. 67, 39–42
Garcia, P.; Pérez de la Vega, M.; Benito, C. (1982): The inheritance of rye seed peroxidases. Theor. Appl. Genet. 61, 341–351
Hirano, H.; Naganuma, K. (1979): Inheritance of peroxidase isozymes in mulberry (Morus ssp.) Euphytica 28, 73–79
Immer, F.R. (1930): Formulae and tables for calculating linkage intensities. Genetics 15, 81–98
Jong, J.H. de; Bock, T.S.M. de (1978): Use of haploids of Beta vulgaris L. for the study of orcein and giemsa stained chromosomes. Euphytica 27, 41–47
Maizonnier, D. (1976): Etude cytogénétique de variations chromosomiques naturelles ou induites chez Petunia hybrida Hort. Thése d'Etat. Univ. Dijon (France)
Maizonnier, D.; Moessner, A. (1979): Localization of the linkage groups on the seven chromosomes of the Petunia hybrida genome. Genetica 51, 143–148
Mather, K. (1938): The measurement of linkage in heredity. London: Methuen & Co.
Pai, C.; Endo, T.; Oka, H.I. (1973): Genic analysis for peroxidase isozymes and their organ specificity in Oryza perennis and O. sativa. Can. J. Genet. Cytol. 15, 845–853
Quiros, C.F.; Morgan, K. (1981): Peroxidase and leucineaminopeptidase in diploid Medicago species closely related to alfalfa: multiple gene loci, multiple allelism and linkage. Theor. Appl. Genet. 60, 221–228
Rick, C.M.; Tanksley, S.D.; Fobes, J. (1979): A pseudo duplication in Lycopersicon pimpinellifolium. Proc. Natl. Acad. Sci. USA 76, 3435–3439
Scandalios, J.G. (1974): Isozymes in development and differentiation. Ann. Rev. Plant Physiol. 25, 225–258
Siciliano, M.J.; Shaw C.R. (1976): Separation and visualization of enzymes on gels. In: Chromatographic and electrophoretic techniques, vol. 2. Zone electrophoresis (ed. Smith, I.), pp. 185–209. London: Heinemann
Tanksley, S.D.; Rick, C.M. (1980): Isozyme gene linkage map of the tomato: applications in genetics and breeding. Theor. Appl. Genet. 57, 161–170
Wiering, H. (1974): Genetics of flower colour in Petunia hybrida Hort. Genen Phaenen 17, 117–134
Woodman, J.C.; Freeling, M. (1981): Identification of a genetic element that controls the organ-specific expression of Adh1 in maize. Genetics 98, 357–378
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Communicated by H. F. Linskens
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van den Berg, B.M., Hendriks, T. & Wijsman, H.J.W. Genetics of the peroxidase isoenzymes in Petunia . Theoret. Appl. Genetics 64, 75–81 (1982). https://doi.org/10.1007/BF00303655
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DOI: https://doi.org/10.1007/BF00303655