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Evaluation of isozyme systems in Citrus to facilitate identification of fusion products

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Summary

Ten isozymes were analyzed in nucellar calli of nine Citrus species and cultivars and roots of the corresponding apomictic seedlings. The zymograms obtained can be divided into three groups: a) isozyme patterns similar in both calli and roots, b) isozyme patterns similar in calli but variable in roots, and c) isozyme patterns variable in both calli and roots. Analysis of these ten isozyme systems may facilitate identification of fusion products in Citrus.

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Literature

  • Brewbaker, J.L. (1971): Pollen enzymes and isoenzymes. In: Pollen: development and physiology (ed. Heslop-Harrison, J.), pp. 156–170. London: Butterworth

    Google Scholar 

  • Brown, A.H.D.; Nevo, E.; Zohary, D.; Dagan, O. (1978): Genetic variation in natural populations of wild barley (Hordeum spontaneum). Genetika 49, 97–108

    Google Scholar 

  • Button, J.; Vardi, A.; Spiegel-Roy, P. (1976): Root peroxidase isoenzymes in Citrus breeding. Theor. Appl. Genet. 47, 119–123

    Google Scholar 

  • Desborough, S.; Peloquin, S.J. (1967): Esterase isoenzymes from Solanum tubers. Phytochemistry 6, 989–994

    Google Scholar 

  • Desborough, S.; Peloquin, S.J. (1968): Potato variety identijication by use of electrophoretic patterns of tubers proteins of enzymes. J. Exp. Bot. 18, 100–109

    Google Scholar 

  • Esen, A.; Soost, R.K. (1976): Peroxidase polymorphism in Citrus. J. Hered. 67, 199–203

    Google Scholar 

  • Iglesias, L.; Lima, H.; Simon, J.P. (1974): Isoenzyme identification of zygotic and nucellar seedlings in Citrus. J. Hered. 65, 81–84

    Google Scholar 

  • Lönnendonker, N.; Schieder, O. (1980): Amylase isoenzymes of the genus Datura as a simple method for an early identification of somatic hybrids. Plant. Sci. Lett. 17, 135–139

    Google Scholar 

  • Lo Schiavo, F.; Mela, L.; Nuti Rouchi, V.; Terzi, M. (1980): Electrophoretic mobility of isozymes from different plant species and its possible use in identifying cell hybrids. Plant. Sci. Lett. 18, 45–55

    Google Scholar 

  • Loukas, M.; Pontikis, C.A. (1979): Pollen isozyme polymorphism in types of Pistacia vera and related species as an aid in taxonomy. J. Hortic. Sci. 54, 95–102

    Google Scholar 

  • Matthews, P.; Williams; H. (1973): Peroxidase zymograms of field beans (Phaseolus vulgaris), pp. 39– 40. 64th A. Rep. Tech. Innes Inst. Norwich

  • Maurer, H.R. (1971): Disc electrophoresis and related technique of polyacrylamide gel electrophoresis. Berlin: W. de Gruyter

    Google Scholar 

  • McCown, B.H.; Beck, G.E.; Hall, T.C. (1969): The hardening response of three clones of Dianthus and the corresponding complement of peroxidase isoenzymes. J. Am. Soc. Hortic. Sci. 94, 691–693

    Google Scholar 

  • McCown, B.H.; McCown, D.D.; Beck, G.E.; Hall, T.C. (1970): Isoenzyme complements of Dianthus callus cultures: influence of light and temperature. Am. J. Bot. 57, 148–152

    Google Scholar 

  • Menczel, L.; Lazav, G.; Maliga, P. (1978): Isolation of somatic hybrids by cloning Nicotiana heterokaryons in nurse cultures. Planta 143, 29–32

    Google Scholar 

  • Murashige, T.; Tucker, D.P.H. (1969): Growth factor requirements of Citrus tissue culture. In: Proc. 1st Int. Citrus Symp. (ed. Chapman, H.D.), pp. 1155–1161. Riverside: Univ. of California

    Google Scholar 

  • Pierce, L.C.; Brewbaker, J.L. (1973): Application of isozyme analysis in horticultural science. Hortic. Sci. 8, 17–22

    Google Scholar 

  • Scandalios, J.G. (1974): Isozymes in development and differentiation. Annu. Rev. Plant Physiol. 25, 225–258

    Google Scholar 

  • Scandalios, J.G.; Sorenson, J.C. (1977): Isozymes in plant tissue culture. In: Plant cell, tissue and organ culture (eds. Reinert, J.; Bajaj, Y.P.S.), pp. 719–730. Berlin, Heidelberg, New York: Springer

    Google Scholar 

  • Shannon, L.M. (1968): Plant isoenzymes. Annu. Rev. Plant Physiol. 19, 187–210

    Google Scholar 

  • Siegel, B.Z.; Galston, A.W. (1967): The isoperoxidases of Pisum sativum. Plant Physiol. 42, 221–226

    Google Scholar 

  • Smith, H.H.; Hamil, D.E.; Weaver, E.A.; Thompson, K.W. (1970): Multiple molecular forms of peroxidasea and esterases among Nicotiana species and amphiploids. J. Hered. 61, 203–214

    Google Scholar 

  • Spiegel-Roy, P.; Vardi, A.; Shani, A. (1977): Peroxidase isozymes as a tool for early separation of nucellar and zygotic Citrus seedlings. Proc. Int. Soc. Citriculture 2, 619–624

    Google Scholar 

  • Torres, A.M.; Diedenhofen, U.; Bergh, B.O.; Knight, R.J. (1978a): Enzyme polymorphism as genetic markers in the avocado. Am. J. Bot. 65, 134–139

    Google Scholar 

  • Torres, A.M.; Soost, R.K.; Diendenhofen, U. (1978b): Leaf isozymes as genetic markers in Citurs. Am. J. Bot. 65, 869–881

    Google Scholar 

  • Vardi, A.; Spiegel-Roy, P.; Galun, E. (1982): Plant regeneration from Citrus protoplasts: variability in methodological requirements among cultivars and species. Theor. Appl. Genet. 62, 171–176

    Google Scholar 

  • Wolfe, W.H. (1976): Identification of grape varieties by isozyme banding patterns. Am. J. End. Vitic. 27, 68–73

    Google Scholar 

  • Zeleneva, I.V.; Khavkin, E.E. (1980): Rearrangement of enzyme patterns in maize callus and suspension cultures. Planta 148, 108–115

    Google Scholar 

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Communicated by R. Riley

Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. No. 354-E, 1982 series

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Ben-Hayyim, G., Shani, A. & Vardi, A. Evaluation of isozyme systems in Citrus to facilitate identification of fusion products. Theoret. Appl. Genetics 64, 1–5 (1982). https://doi.org/10.1007/BF00303641

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