Abstract
The method of adsorption of drugs on Amberlite XAD-2 resin, followed by differential elution was under study during 1 1/2 year of routine use. The method allows a separation of acidic and basic drugs and — due to acid hydrolysis step before adsorption — assures better recovery of conjugated and proteinbound drugs. The amounts of various drugs, found in autopsy cases by the XAD-2 method were usually higher than those found by solvent extraction. The method applied requires 20 g of sample (biofluid or tissue) for general toxicological analysis.
Zusammenfassung
Die Methode der Arzneiadsorption mittels des XAD-2-Harzes mit der nachfolgenden differenzierten Elution wurde in 1 1/2 Jahre dauerndem Gebrauch untersucht. Diese Methode ermöglicht es sauere und basische Arzneien abzufremmen und ergibt eine bessere Ausbeute für die gebundene Drogen, weil der sauren Hydrolyse die Adsorption vorangeht. Die Ausbeute der bei letalen Vergiftungen anhand der XAD-2-Methode ausgemittelten Arzneisubstanzen war in der Regel höher als bei der Extraktion mit Lösungsmitteln. Die hier verwendete Methode erfordert 20 g des biologischen Materials für eine allgemeine toxikologische Analyse.
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Bogusz, M., Gierz, J. & Białka, J. Isolation of drugs from autopsy material by XAD-2 adsorption-elution technique a routine procedure. Arch. Toxicol. 41, 153–162 (1978). https://doi.org/10.1007/BF00302527
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DOI: https://doi.org/10.1007/BF00302527