Summary
In order to improve L-malic acid productivity by Brevibacterium flavum immobilized with ϰ-carrageenan, addition of Chinese gallotannin to the immobilization medium was investigated. As the results show, the optimal concentration of Chinese gallotannin was 0.1% (w/v). Fumarase activity and the stability of this improved preparation were higher than in one with only ϰ-carrageenan. Addition of Chinese gallotannin was more advantageous to stability towards ethanol than addition of polyethyleneimine. The L-malic acid productivity of the immobilized cells at 37°C was 42.2 kg/h per 1,000 l column, and increased threefold compared with that of B. flavum immobilized with only ϰ-carrageenan, and was 25 times that of B. ammoniagenes immobilized with polyacrylamide. Persimmon tannin also increased the stability of fumarase.
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Takata, I., Tosa, T. & Chibata, I. Stability of fumarase activity of Brevibacterium flavum immobilized with ϰ-carrageenan and Chinese gallotannin. Appl Microbiol Biotechnol 19, 85–90 (1984). https://doi.org/10.1007/BF00302446
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DOI: https://doi.org/10.1007/BF00302446