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A rapid chromatographic procedure for the determination of adriamycin, daunomycin and their 13-OH metabolites adriamycinol and daunomycinol

  • Original Articles
  • Anthracyclines, HPLC Determination
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Summary

A rapid chromatographic procedure for the quantitative determination of the anthracycline antibiotics adriamycin and daunorubicin and their chief metabolites adriamycinol and daunorubicinol in plasma and urine is described. The extraction is performed using SEP-PAK silica cartridges. After filtration the eluate is chromatographed on a reversed-phase column.

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References

  1. Aszalos A (1984) Analysis of antitumor antibiotics by high pressure liquid chromatography (HPLC). J Liq Chromatogr 7 (S-1): 69

    Google Scholar 

  2. Carter SK (1975) Adriamycin—a review. J Natl Cancer Inst 55: 1265

    Google Scholar 

  3. Davis HL, Davis TE (1979) Daunorubicin and adriamycin in cancer treatment: an analysis of their roles and limitations. Cancer Treat Rep 63: 89

    Google Scholar 

  4. Eksborg S, Ehrsson H, Andersson I, Beran M (1978) Liquid chromatographic determination of daunorubicin and daunorubicinol in plasma of leukemic patients. J Chromatogr 153: 211

    Google Scholar 

  5. Erttmann R, Grandt U, Erb N, Landbeck G (1986) Zur Pharmakokinetik von Doxorubicin. Ergeb Paed Onkol 10 (in press)

  6. Hulhoven R, Desager JP (1976) Quantitative determination of low levels of daunomycin and daunomycinol in plasma by high-performance liquid chromatography. J Chromatogr 125: 369

    Google Scholar 

  7. Pierce RN, Jatlow PI (1979) Measurement of adriamycin (doxorubicin) and its metabolites in human plasma using reversed-phase high-performance liquid chromatography and fluorescence detection. J Chromatogr 164: 471

    Google Scholar 

  8. Robert J (1980) Extraction of anthracyclines from biological fluids for HPLC evaluation. J Liq Chromatogr 3: 1561

    Google Scholar 

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Authors and Affiliations

  1. Children's Hospital, University of Hamburg, Department of Hematology and Oncology, Martinistraße 52, D-2000, Hamburg 20, Federal Republic of Germany

    N. Erb, R. Erttmann & G. Landbeck

Authors
  1. N. Erb
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  2. R. Erttmann
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  3. G. Landbeck
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Additional information

The authors wish to thank the Werner Otto Stiftung, Hamburg, and the Hamburger Landesverband für Krebsbekämpfung und Krebsforschung e. V. for their generous financial support

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Erb, N., Erttmann, R. & Landbeck, G. A rapid chromatographic procedure for the determination of adriamycin, daunomycin and their 13-OH metabolites adriamycinol and daunomycinol. Cancer Chemother. Pharmacol. 17, 53–55 (1986). https://doi.org/10.1007/BF00299866

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  • DOI: https://doi.org/10.1007/BF00299866

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