Abstract
The distribution of albumin throughout enamel development in the rat mandibular incisor was investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and Western blotting employing an anti-rat albumin antibody. Intact albumin was detectable at all stages of enamel development but was most evident during late secretion/transition. Its concentration was subsequently reduced during the maturation stage. Albumin degradation products appeared during the transition/early maturation stage indicating that albumin breakdown preceded its removal. As albumin inhibits apatite crystal growth, its degradation and removal may be a necessary prerequisite for normal enamel crystal growth, perhaps reflecting a general mechanism for removal of residual endogenous matrix or adventitious crystal growth inhibitors. Additional studies revealed that the maturation stage was particularly susceptible to albumin influx postmortem. Albumin could therefore form part of the natural crystal growth control process, which, if not removed, could hamper maturation and lead to white spot hypoplasias.
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Termine JD, Belcourt AB, Christner PJ, Conn KM, Nylen MU (1980) Properties of dissociatively extracted fetal tooth matrix proteins I. Principle molecular species in developing bovine enamel. J Biol Chem 255(20):9760–9768
Ogata Y, Shimakawa H, Sasaki S (1988) Purification, characterisation, and biosynthesis of bovine enamelins. Calcif Tissue Int 43:389–399
Limeback H, Sakarya H, Chu W, Mackinnon M (1989) Serum albumin and its acid hydrolysis peptides dominate preparations of mineral-bound enamel proteins. J Bone Miner Res 4(2):235–241
Strawich E, Glimcher MJ (1990) Tooth enamelins identified mainly as serum proteins. Major enamelin is albumin. J Biochem 191:47–56
Fukae M, Tanabe T (1987) Nonamelogenin components of porcine enamel in the protein fraction free from the enamel crystals. Calcif Tissue Int 40:286–293
Robinson C, Lowe NR, Wetherell JA (1975) Amino acid composition, distribution and origin of “tuft” protein in human and bovine dental enamel. Arch Oral Biol 20:29–42
Deutsch D, Palmon A, Fisher LW, Kolodny N, Termine JD, Young MF (1991) Sequencing of bovine enamelin (“tuftelin”), a novel acidic enamel protein. J Biol Chem 266(24):16021–16028
Overall CM, Limeback H (1988) Identification and characterisation of enamel proteinases isolated from developing enamel. Biochem J 256:965–972
DenBesten PK, Heffernan LM (1989) Separation by polyacrylamide gel electrophoresis of multiple proteases in rat and bovine enamel. Arch Oral Biol 34(6):399–404
Robinson C, Shore RC, Kirkham J, Stonehouse NJ (1990) Extracellular processing of enamel matrix proteins and the control of crystal growth. J Biol Buccale 18:355–361
Garnett J, Dieppe P (1990) The effects of serum and human albumin on calcium hydroxyapatite crystal growth. Biochem J 266:836–868
Robinson C, Kirkham J, Brookes SJ, Shore RC (1992) The role of albumin in developing rodent dental enamel: a possible explanation for white spot hypoplasia. J Dent Res 71(6):1270–1274
Couwenhoven RI, Davis C, Snead ML (1989) Mouse ameloblasts do not transcribe the albumin gene. Calcif Tissue Int 45:367–371
Hiller CR, Robinson C, Wetherell JA (1975) Variations in the composition of developing rat incisor enamel. Calcif Tissue Res 18:1–12
Robinson C, Hiller CR, Weatherell JA (1974) Uptake of 32P-labelled phosphate into developing rat incisor enamel. Calcif Tissue Res 15:143–152
Robinson C, Briggs HD, Atkinson PJ (1981) Histology of enamel organ and chemical composition of adjacent enamel in rat incisors. Calcif Tissue Int 33:513–520
Gorbunoff MJ (1984) The interaction of proteins with hydroxyapatite I. Role of protein charge and structure. Anal Biochem 136:425–432
Rykke M, Gunnar R, Sönju T (1990) Effect of sodium lauryl sulfate on protein adsorption to hydroxyapatite in vitro and on pellicle formation in vivo. Scand J Dent Res 98:135–143
Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range 1 to 100 KDa. Anal Biochem 166:368–379
Towbin H, Staehelin T, Gordon J (1979) Electrotransfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc Natl Acad Sci USA 76:4350–4354
Robinson C, Kirkham J (1984) Dynamics of amelogenesis as revealed by protein compositional studies. In: Butler WT (ed) The chemistry and biology of mineralised tissues. Proc 2nd Int Conf on the Chemistry and Biology of Mineralised Tissues, Gulf Shores Alabama, pp 248–263
Kallenbach E (1967) Cell architecture in the papillary layer of rat incisor enamel organ at the stage of enamel maturation. Anat Rec 157:685–698
Gibson CW, Lally E, Herold RC, Decker S, Brinster RL, Sandgren EP (1992) Odontogenic tumors in mice carrying albumin-myc and albumin-ras transgenes. Calcif Tissue Int 51:162–167
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Robinson, C., Brookes, S.J., Kirkham, J. et al. Uptake and metabolism of albumin by rodent incisor enamel In vivo and postmortem: Implications for control of mineralization by albumin. Calcif Tissue Int 55, 467–472 (1994). https://doi.org/10.1007/BF00298561
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DOI: https://doi.org/10.1007/BF00298561