Abstract
We examined the role of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) during the activation of peripheral blood mononuclear cells (PBMCs) by the human renal carcinoma cell line CaKi-1. ICAM-1 antigen expression was induced on CaKi-1 cells by incubation with either phorbol-12-myristate 13 acetate (PMA) or interferongamma (IFN-γ). Following a thorough washout of PMA and IFN-γ and subsequent paraformaldehyde fixation, CaKi-1 cell monolayers were cocultered with allogenic PBMCs. While PMA-treated CaKi-1 cells induced PBMC proliferation and interleukin-2 receptor antigen expression, this was not the case for control of IFN-γ-treated CaKi-1 cells. Furthermore, the induced PBMC proliferation was inhibited by specific monoclonal antibodies against ICAM-1 and LFA-1. Finally, although PMA induced human leukocyte antigen (HLA)-A, B, C antigen expression on CaKi-1 cells, a monoclonal anti-body against this antigen did not inhibit PBMC proliferation. We conclude that PMA can modulate CaKi-1 cells to stimulate allogenic PBMC proliferation in an ICAM-1/LFA-1 dependent, but HLA-A, B, C-independent, fashion. This stimulation might reside in the long-term activation of protein kinase C, induced by PMA.
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Azuma A, Yagita H, Matsuda H, Okumura K, Niitani H (1992) Induction of intercellular adhesion molecule 1 on small lung carcinoma cell lines by γ-interferon enhances spontaneous and bispecific anti-CD 3 x antitumour antibody directed lymphokine-activated killer cell cytotoxicity. Cancer Res 52:4890
Balch CM, Riley LB, Bae Y-J, Salmeron MA, Platsoucas CD, Eschenbach AC von, Itoh K (1990) Patterns of human tumor-infiltrating lymphocytes in 120 human cancers. Arch Surg 125:200
Belldegruun A, Mull LM, Rosenberg SA (1988) Interleukin 2 expanded tumour-infiltrating lymphocytes in human renal cell cancer: Isolation, characterization, and antitumour activity. Cancer Res 48:206
Boyum A (1968) A one stage process for the isolation of granulocytes and lymphocytes from human blood: General properties of white blood cells in a 1 g gravity field. Scand J Clin Lab Invest 21 [Suppl 97]:51
DeKernion J, Ramming K, Smith R (1978) The natural history of metastatic renal cell carcinoma: a computer analysis. J Urol 120:148
Fogh J, Trempe G (1975) Human tumour cells in vivo. Plenum, New York, p 115
Goedebebuure PS, Braakman E, Segal DM, Vreugdenhil R, Bolhuis RLM (1990) Lymphocyte leukocyte function-associated antigen 1 interacting with target cell intercellular adhesion molecule 1 co-activates cytolysis triggered via CD-16 or the receptor involved in major histocompatibility antigen unrestricted lysis. Int Immunol 2:1213
Goepel M, Rübben H (1991) Adjuvant therapy in renal cancer. World J Urol 9:323
Hansen AB, Andersen CB (1993) Comparison of the effects of tumour necrosis factor α stimulation and phorbol ester treatment on the immunocytochemical staining of intercellular adhesion molecule 1 in human renal carcinoma cell cultures. Virchows Arch B Cell Pathol 63:107
Hansen AB, Bouchelouche PN, Giese BN, Andersen CB (1993) Role of protein kinase C during interferon-γ and phorbol esterstimulated immunocytochemical expression of ICAM-1 in human renal carcinoma cells. APMIS 101:437
Hansen AB, Bouchelouche PN, Lillevang AT, Andersen CB (1994) Interferon-gamma increases cellular calcium ion concentration and inosital 1,4,5-triphosphate formation in human renal carcinoma cells: relation to ICAM-1 antigen expression. Br J Cancer 69:291
Hansen AB, Lillevang ST, Andersen CB (1994) Stimulation of intercellular adhesion molecule-1 (ICAM-1) antigen expression and shedding by interferon-gamma and phorbol ester in human renal carcinoma cell cultures: Relation to peripheral blood mononuclear cell adhesion. Urol Res 22:85
Hayakawa K, Morita T, Augustus LB, Eschenbach AC von, Itoh K (1992) Human renal-cell carcinoma cells are able to active natural killer cells. Int J Cancer 51:290
Jackson AM, Alexandrov AB, Gribben SC, Esuvarnathan K, James K (1993) Expression and shedding of ICAM-1 in bladder cancer and its immunotherapy. Int J Cancer 55:921
Kanamaru H, Yoshida O (1989) Assessment of in vitro lymphokine activated killer (LAK) cell activity against renal cancer cell lines and its suppression by serum factor crystal violet assay. Urol Res 17:259
Kurt-Jones EA, Virgin HW, Unanue ER (1986) In vivo and in vitro expression of macrophage membrane interleukin 1 in response to soluble and particulate stimuli. J Immunol 137: 10
Makgoba MW, Bernard A, Sandeers ME (1992) Cell adhesion/signalling: biology and clinical applications. Eur J Clin Invest 22:443
Male D, Champion B, Cooke A (1987) Advanced immunology. Grower Medical, London, p 4.1
Naganuma H, Kiessling R, Patarroyo M, Hansson M, Handgretinger R, Grönberg A (1991) Increased susceptibility of IFN-γ-treated neuroblastoma cells to lysis by lymphokine-activated killer cells: participation of ICAM-1 induction on target cells. Int J Cancer 47:527
Nishizuka Y (1989) Studies and prospectives of the protein kinase C family for cellular regulation. Cancer 63:1892
Patarroyo M, Makgoba (1989) Leucocyte adhesion to cells: molecular basis, physiological relevance, and abnormalities. Scand J Immunol 30:129
Rosenberg SA, Lotze MT, Muul LM, Leitman S, Chang AE, Ettinghausen SE, Matory YL, Skibber JM, Shiloni E, Vetto JT, Seipp CA, Simpson C, Reichert CM (1985) Observation on the systemic administration of autologous lymphokine activated killer cells and recombinant interleukin-2 to patients with metastatic cancer. N Engl J Med 313:1485
Simon JC, Cruz PD Jr, Bergstresser PR, Davis LS, Tigelaar RE (1991) Phorbol myristate-activated keratinocytes stimulate proliferation of resting peripheral blood mononuclear lymphocytes via a MHC-independent, but protein kinase C and intercellular molecule-1-dependent, mechanism. J Immuno 146:476
Tomita Y, Watanabe H, Kobayashi H, Nishiyama T, Tsuji S, Fujiwara M, Sato S (1992) Interferon γ but not tumour necrosis factor α decreases susceptibility of human renal cell cancer cell lines to lymphokine-activated killer cells. Cancer Immunol Immunother 35:381
Zinkernagel RM, Doherty OC (1979) MHC cytotoxic T-cell: Studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction specificity, function and responsiveness. Adv Immunol 27:51
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Hansen, A.B., Andersen, C.B. Role of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-1 (LFA-1) in peripheral blood mononuclear cell activation by human renal carcinoma cells. Urol. Res. 22, 309–315 (1994). https://doi.org/10.1007/BF00297201
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DOI: https://doi.org/10.1007/BF00297201