Abstract
Spin trapping in vivo was first achieved in the author's laboratory and is shown to be a feasible method for demonstrating that highly reactive free radical intermediates are generated in the tissues of intact animals as a result of the exposure to certain toxic compounds and to ionizing radiation. The method is based on the property of spin trapping agents (nitrones) to react readily with reactive free radicals to produce stable radical adducts at the site of their origin in target organs. The radical adducts can then be detected by electron spin resonance spectroscopy to determine the intensity of radical production (i.e., number of radicals which were trapped), and, in most cases, identify the nature of the radical that was produced. The type of spin trapping agent employed determines the type of radicals which can be trapped and, at this stage of development of the technique, the number of useful in vivo trapping agents is rather limited.
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Augusto O, DuPlessis LR, Weingrill CL (1985) Spin-trapping of methyl radical in the oxidative metabolism of 1,2-Dimethylydrazine. Biochem Biophys Res Commun 126: 853–858
Floyd RA, Soong LM, Stuart MA, Reigh DL (1978) Spin trapping of free radicals produced from nitrosoamine carcinogens. Photochem Photobiol 28: 857–862
Gregus Z, Varga F (1985) Role of glutathione and hepatic glutathione S-transferase in the biliary excretion of methyl mercury, cadmium and zinc: a study with enzyme inducers and glutathione depletors. Acta Pharmacol Toxicol (Copenh) 56: 398–403
Harbour JR, Bolton JR (1978) The involvement of the hydroxyl radical in the destructive photooxidation of chlorophyls in vivo and in vitro. Photochem Photobiol 28: 231–234
Janzen EG, Stronks HJ, DuBose CM, Poyer JL, McCay PB (1985) Chemistry and Biology of spin-trapping radicals associated with halocarbon metabolism in vitro and in vivo. Environ Health Perspect 64: 151–170
Kalyanaraman B, Mason RP, Perez-Reyes E, Chignell CF, Wolf CR, Philpot RM (1979) Characterization of the free radical formed in aerobic microsomal incubations containing carbon tetrachloride and NADPH. Biochem Biophys Res Commun 89: 1065–1072
Kubow S, DuBose CM, Janzen EG, Carlson JR, Bray TM (1983) The spin trapping of enzymatically and chemically catalyzed free radicals from indolic compounds. Biochem Biophys Res Commun 114: 168–174
Lai EK, Crossley C, Sridhar R, Misra HP, Janzen EG, McCay PB (1986) In vivo spin trapping of free radicals generated in brain, spleen, and liver during gamma radiation of mice. Arch Biochem Biophys 244: 156–160
Lai C-S, Piette LH (1977) Hydroxyl radical production involved in lipid peroxidation of rat liver microsomes. Biochem Biophys Res Commun 78 (1): 51–59
Poyer JL, Floyd RA, McCay PB, Janzen EG, Davis ER (1978) Spin trapping of the trichloromethyl radicals produced during enzymic NADPH oxidation in the presence of carbon tetrachloride or carbon Bromotrichloromethane. Biochem Biophys Acta 539: 402–409
Recknagel RO, Glende EA, Hruszkewycz AM (1977) Chemical mechanisms in carbon tetrachloride toxicity. Free Rad Biol Vol III: 97–132
Reynolds ES, Moslen MT (1980) Free radical damage in liver. Free Rad Biol IV: 49–94
Rosen GM, Kloss MW, Rauckman EJ (1982) Initiation of in vitro lipid peroxidation by N-Hydroxynorcocaine and norcocaine nitroxide. Mol Pharmacol 22: 529–531
Rosen GM, Rauckman EJ (1979) Spin trapping of the primary radical involved in the activation of the carcinogen N-Hydroxy-2-acetylaminofluorene by cumene Hydroperoxide-hematin. Mol Pharmacol 17: 233–238
Saprin AN, Piette LH (1977) Spin trapping and its application in the study of lipid peroxidation and free radical production with liver microsomes. Arch Biochem Biophys 180: 480–492
Sealy RC, Swartz HM, Olive PL (1978) Electron spin resonancespin trapping. Detection of superoxide formation during aerobic microsomal reduction of nitro-compounds. Biochem Biophys Res Commun 82: 680–684
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Dedicated to Professor Dr. med. Herbert Remmer on the occasion of his 65th birthday
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McCay, P.B. Application of ESR spectroscopy in toxicology. Arch Toxicol 60, 133–137 (1987). https://doi.org/10.1007/BF00296966
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DOI: https://doi.org/10.1007/BF00296966