Abstract
The effect of combined administration of ethanol and manganese on the brain tissue of rats was investigated to evaluate the role of alcohol ingestion in inducing susceptibility to manganese poisoning. Ethanol and manganese alone and the combination of the two were administered orally daily to the rats for 30 days. Almost identical increase in the brain contents of manganese in rats receiving the metal alone and in combination with ethanol indicates that ethanol administration does not influence the accumulation of manganese in that organ. The copper contents of brain also increased to almost the same extent in these two groups. Synergistic effect of ethanol and manganese was noticed on increasing the activity of ATPase and RNase while marked antagonistic effect was observed on the activity of MAO. The mechanism and the significance of these neurochemical alterations occurring after the administration of ethanol and manganese have been discussed.
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Chandra, S. V.: Histological and histochemical changes in experimental manganese encephalopathy in rabbits. Arch. Toxicol. (Berl.) 29, 29–38 (1972)
Chandra, S. V., Shukla, G. S.: Role of iron deficiency in inducing susceptibility to manganese toxicity. Arch. Toxicol. (Berl.) 35, 319–323 (1976)
Chandra, S. V., Shukla, G. S.: Manganese encephalopathy in growing rats. Environ. Res. 15, 28–37 (1978)
Chandra, S. V., Seth, P. K., Mankeshwar, J. K.: Manganese poisoning: Clinical and biochemical observations. Environ. Res. 7, 374–380 (1974)
Christian, Gary D., Feldman, F. J.: Atomic absorption spectroscopy. New York: Wiley-Interscience A Division of John Wiley and Sons, 1970
Cotzias, G. C.: Manganese in health and disease. Physiol. Rev. 38, 503–532 (1958)
Cumings, J. N.: In: Brain 71, 410 (1948). Quoted from Greenfield's Neuropathology, p. 270. London: Edward Arnold Ltd. 1965
Fiske, C. M., SubbaRow, V.: Hawk's physiological chemistry. Oser, B. L. (ed.), 14th ed. p. 1113. London: McGraw-Hill 1925
Fisher, R. A.: Statistical methods for research workers, 11th ed. London: Oliver & Boyd 1950
Greenfield's Neuropathology, p. 270. London: Edward Arnold Ltd. 1965
Hestrin, S.: The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine and its analytical application. J. Biol. Chem. 180, 249–261 (1949)
Lowry, O. H., Orsebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
McDonald, R. M.: Ribonucleases. In: methods in enzymology, vol. II. Colowick, S. P., Kaplan, N. O. (eds.), p. 427. New York: Academic Press 1955
McEven, Ch. M., Jr.: Monoamine oxidase (rabbit serum) In: Methods in enzymology, vol. XVII B. Tabor, H., White Tabor, C. (eds.), pp. 686–692. London, New York: Academic Press 1971
McManus, J. F. A., Mowry, R. W.: Staining methods: Histologic and histochemical. In: Heobar medical division. New York: Evanston; London: Harper & Row 1965
Mella, H.: The experimental production of basal ganglion symptomatology in Macacus Rhesus. Arch. Neurol. Psychiat. (Chic.) 11, 405 (1924)
Mena, I., Horiachi, K., Burke, K., Cotzias, G. C.: Chronic manganese poisoning, individual susceptibility and absorption of iron. Neurology 19, 1000–1006 (1969)
Mustafa, S. J., Tewari, C. P.: Latent adenosine deaminase in mouse brain. Biochim. Biophys. Acta 198, 93–100 (1970)
Pentschew, A., Ebner, F. F., Kovatch, R. M.: Experimental manganese encephalopathy in monkeys. J. Neuropathol. Exp. Neurol. 22, 488–499 (1963)
Reitman, S., Frankel, S.: A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28, 56–63 (1957)
Rodier, J.: Manganese poisoning in Moroccan Miners. Br. J. Ind. Med. 12, 21–35 (1955)
Roman, W., Ruys, J.: The colorimetric estimation of arginase in serum. In: Clinical enzymology, vol. II, p. 121–128. (Eds. J. Frei and M. Jemelin). Baltimore, London, Tokyo: University Park Press 1969
Schafer, D. F., Stephenson, D. V., Barak A. J., Sorrell, M. F.: Effects of ethanol on the transport of manganese by small intestine of the rat. J. Nutr. 104, 101–104 (1974)
Schroeder, H. A., Mason, A. P.: Interactions of trace metals in rat tissues. Cadmium, nickel with zinc, chromium, copper and manganese. J. Nutr. 104, 167–178 (1974)
Seth, P. K., Tangari, K. K.: Biochemical effects of newer salicylic acid congeners. J. Pharm. Pharmacol. 18, 831–833 (1966)
Shukla, G. S., Chandra, S. V., Seth, P. K.: Effect of manganese on the levels of DNA, RNA, DNase and RNase in cerebrum, cerebellum and rest of brain regions of rat. Acta Pharmacol. Toxicol. (Kbh.) 39, 562–569 (1976)
Singh, J., Husain, R., Tandon, S. K., Seth, P. K., Chandra, S. V.: Biochemical and histopathological alterations in early manganese toxicity in rats. Environ. Physiol. Biochem. 4, 16–23 (1974)
Slater, E. C., Bonner, W. D.: The effects of fluoride on the succinic oxidase system. Biochem. J. 52, 185–196 (1952)
Sung, S. C.: Deoxyribonucleases from rat brain. J. Neurochem. 15, 477–481 (1968)
Tolonen, M.: Industrial toxicology of manganese. Wk. Environ. Hlth. 9, 53–60 (1972)
Wooton, I. D. P.: Microanalysis in medical biochemistry, 4th ed., p. 107. London: Churchill 1964
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Singh, S., Shukla, G.S., Srivastava, R.S. et al. The interaction between ethanol and manganese in rat brain. Arch. Toxicol. 41, 307–316 (1979). https://doi.org/10.1007/BF00296901
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DOI: https://doi.org/10.1007/BF00296901