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Glomeruloid blood vessels in ethylnitrosourea-induced rat gliomas

Histological and immunohistochemical studies

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Summary

Glomeruloid blood vessels (GBVs), a characteristic histological feature of most human malignant gliomas, were recognized with high incidence in autochthonous rat gliomas induced by transplacental administration of ethylnitrosourea. To evaluate some of the biological properties of these GBVs, we carried out a study using histological methods and immunohistochemical staining for glial fibrillary acidic protein, factor VIII-related antigen (VIII Ag) and bromodeoxyuridine (BrdUrd). Of 22 animals with large, massively growing gliomas in the CNS, GBVs including conglomerate aggregations of small blood vessels with endothelial hyperplasia and strong VIII Ag expression were observed in 13 large gliomas histologically consisting of primitive neuroepithelial neoplasms (PNN; so called ependymoma) and mixed-type gliomas in combination with astrocytoma and PNN or anaplastic astrocytoma. The anaplastic gliomas in our series were devoid of GBVs. These findings indicate that GBV formation takes place in a histological variety of experimental gliomas. Furthermore, the GBVs were frequently associated with the vasculo-mesenchymal stroma in the parent gliomas, suggesting an intimate relationship with the morphogenesis of GBVs. In addition, it was shown that the GBVs had a higher BrdUrd-labelling index than that of other blood vessels in gliomas and also that of neoplastic cells in most parent gliomas, except for anaplastic gliomas. Based on these results, the possible mechanism of GBV morphogenesis is discussed with regard to the roles of macromolecules in the induction and regulation of GBVs.

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Author notes

  1. Y. Yoshida

    Present address: Department of Neuropathology, Institute for Neurological Science, Hirosaki University School of Medicine, Zaifuchou 5, 036, Hirosaki, Japan

Authors and Affiliations

  1. Department of Neuropathology Brain Research Institute, Niigata University, Asahimachi 1, 951, Niigata, Japan

    Y. Yoshida, T. Kumanishi, S. Abe & A. Nishiyama

  2. Department of Pathology, Brain Research Institute, Niigata University, Asahimachi 1, 951, Niigata, Japan

    M. Yamada & K. Hinokuma

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  1. Y. Yoshida
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  2. T. Kumanishi
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  3. S. Abe
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  4. A. Nishiyama
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  5. M. Yamada
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Additional information

Supported in part by grants nos. 63570675 and 01480350 from the Ministry of Education, Science and Culture, Japan, and by a grant from the Ministry of Health and Welfare, Japan

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Yoshida, Y., Kumanishi, T., Abe, S. et al. Glomeruloid blood vessels in ethylnitrosourea-induced rat gliomas. Acta Neuropathol 79, 240–247 (1989). https://doi.org/10.1007/BF00294657

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  • DOI: https://doi.org/10.1007/BF00294657

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