Summary
The binding of fibrinogen with heparin and dextran sulfates, and of antithrombin III with heparin and dextran sulfate were investigated by sedimentation velocity method. From the measurement of fibrinogen-acidic polysaccharide systems, it was confirmed that heparin and low molecular weight dextran sulfate(DSC) forms soluble complexes with fibrinogen, though the amount bound of the latter was rather small compared with high molecular weight dextran sulfate(DSD). Antithrombin III was also found to be bound by heparin and DSC. The numbers of heparin molecules bound to one molecule of fibrinogen and antithrombin III were estimated to be 4.2 and 0.3, respectively.
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Nakanishi, E., Sato, H. & Nakajima, A. Sedimentation study on the binding of fibrinogen or of antithrombin III with acidic polysaccharides including heparin. Polymer Bulletin 24, 429–435 (1990). https://doi.org/10.1007/BF00294097
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DOI: https://doi.org/10.1007/BF00294097