Summary
The location of 339 break points was analyzed in three patients with Fanconi's anemia, using three consecutive stains: ordinary Giemsa, Q-banding, and R-banding. Almost all the breaks seem to take place in the Q bands, using R-banding, and in the R bands, using Q-banding. A very important artifact, varying according to the method used, is thus demonstrated. In fact, the breaks take place in the interbands, between R and Q bands.
The breaks were also localized in relation to sister chromatid exchanges (SCEs), seen after BUDR treatment. There is a clear excess of breaks at places of SCE (29%). This may indicate a possible correlation between breaks and SCEs.
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Bourgeois, C. A.: personal communication (1976)
Buckton, K. E.: Identification with G and R banding of the position of breakage points induced in human chromosomes by “in vitro” irradiation. Int. J. Radiat. Biol. 29, 475–488 (1976)
Caspersson, T., Zech, L., Johansson, C.: Analysis of human metaphase chromosome set by aid of DNA-binding fluorescent agents. Exp. Cell Res. 62, 490–492 (1970)
Caspersson, T., Haglund, U., Lindell, B., Zech, L.: Radiation-induced non-random chromosome breakage. Exp. Cell Res. 75, 541–543 (1972)
Chaganti, R. S. K., Schonberg, S., German, J.: A manifold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes. Proc. nat. Acad. Sci. (Wash.) 71, 4508–4512 (1974)
Dutrillaux, B.: Sur la nature et l'origine des chromosomes humains. Monogr. Ann. Génét., 3, Expansion Scientifique, Paris (1975)
Hayashi, K., Schmid, W.: The rate of sister chromatid exchanges parallel to spontaneous chromosome breakage in Fanconi's anemia and to trenimon-induced aberrations in human lymphocytes and fibroblasts. Hum. Genet. 29, 201–206 (1975)
Holmberg, M., Jonasson, J.: Preferential location of X-ray induced chromosome breakage in the R-bands of human chromosome. Hereditas (Lund) 74, 57–68 (1973)
Jonasson, J., Holmberg, M.: Evidence for an inversion relationship between X-ray induced chromatid and chromosome breakage in human chromosomes. Hereditas (Lund) 75, 259–266 (1973)
Korenberg, J. R., Freedlender, E. F.: Giemsa technique for the detection of sister chromatids exchanges. Chromosoma (Berl.) 48, 355–360 (1974)
Koskull, H. von, Aula, P.: Nonrandom distribution of chromosome breaks in Fanconi's anemia. Cytogenet. Cell Genet. 12, 423–434 (1973)
Latt, S. A., Stettew, G., Juergens, L. A., Buchanan, G. R., Gerald, P. S.: Induction by alkylating agents of sister chromatid exchanges and chromatid breaks in Fanconi's anemia. Proc. nat. Acad. Sci. (Wash.) 72, 4066–4070 (1975)
Paris Conference: Standardization in human cytogenetics. Birth Defects, Original Article Series 8 (1972)
Perry, P., Evans, H. J.: Cytological detection of mutagen-cancerigen exposure by sister chromatid exchanges. Nature 258, 121–125 (1975)
Prieur, M., Dutrillaux, B., Lejeune, J.: Planches descriptives des chromosomes humains (analyse en bandes R et nomenclature selon la Conférence de Paris, 1971). Ann. Génét. 16, 39–46 (1973)
Schmid, W.: Familial constitutional panmyelocytopathy, Fanconi anemia (F.A.).II. A discussion of the cytogenetic findings in F.A. Sem. inv. Hemat. 4, 241–249 (1967)
Schroeder, T. M.: Cytogenetische und cytologische Befunde bei enzymopenischen Panmyelopathien und Pancytopenien. Hum. Genet. 2, 287–316 (1966)
Schroeder, T. M., Anschütz, F., Knopp, A.: Spontane Chromosomenaberrationen bei familiärer Panmyelopathie. Hum. Genet. 1, 194–196 (1964)
Seabright, M.: High resolution studies on the pattern of induced exchanges in the human karyotype. Chromosoma (Berl.) 40, 333–346 (1973)
Sperling, K., Wegner, R. D., Riehm, H., Obe, G.: Frequency and distribution of sister chromatid exchanges in a case of Fanconi anemia. Hum. Genet. 27, 227–230 (1975)
Swift, M. R., Hirschhorn, K.: Fanconi's anemia inherited susceptibility to chromosome breakage in various tissues. Ann. intern. Med. 65, 496–503 (1966)
Wolman, S. R., Swift, M.: Bone-marrow chromosomes in Fanconi's anemia. J. med. Genet. 9, 437–441 (1972)
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Technical assitance: Anne-Marie Fosse and Martine Lombard
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Dutrillaux, B., Couturier, J., Viegas-Péquignot, E. et al. Localization of chromatid breaks in Fanconi's anemia, using three consecutive stains. Hum Genet 37, 65–71 (1977). https://doi.org/10.1007/BF00293773
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DOI: https://doi.org/10.1007/BF00293773