Abstract
The frequency of sister chromatid exchanges (SCEs) has been determined for C band and non-C band regions of chromosomes of the kangaroo rat after staining with the fluorescence plus giemsa (FPG) technique. After one complete round of DNA synthesis in the presence of bromodeoxyuridine (BrdU) staining of the C band regions revealed simple or complex asymmetries between chromatids. After two complete rounds of DNA synthesis in the presence of BrdU “harlequin” chromosomes were observed. Analysis of the distribution of SCE in chromosomes at their 1st and 2nd mitosis showed that relatively few exchanges occur within C band regions, although the frequency of SCEs is high at the junction between C band and non-C band chromosome regions.
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Angell, R.R., Jacobs, P.A.: Lateral asymmetry in Human constitutive heterochromatin. Chromosoma (Berl.) 51, 301–310 (1975)
Bostock, C.J., Christie, S.: Chromosome banding and DNA replication studies on a cell line of Dipodomys merriami. Chromosoma (Berl.) 48, 73–87 (1974)
Bostock, C.J., Prescott, D.M., Hatch, F.T.: Timing of replication of the satellite and main band DNAs in cells of the kangaroo rat (Dipodomys ordii). Exp. Cell Res. 74, 487–495 (1972)
Buckton, K. E.: Identification with G and R banding of the position of breakage points induced in human chromosomes by in vitro X-irradiation. Int. J. Radiat. Biol. (in press, 1976)
Buckton, K.E., O'Riordan, M.L., Jacobs, P.A., Robinson, J.A., Hill, R., Evans, H.J.: C and Q band polymorphisms in the chromosomes of three human populations. Ann. hum. Genet. (in press, 1976)
Carrano, A. V., Wolff, S.: Distribution of sister chromatid exchanges in the euchromatin and heterochromatin of the Indian muntjak. Chromosoma (Berl.) 53, 361–369 (1975)
Fry, K., Poon, R., Whitcome, P., Idriss, J., Salzer, W., Mazrimas, J., Hatch, F.: Nucleotide sequence of HS-β satellite DNA from kangaroo rat Dipodomys ordii. Proc. nat. Acad. Sci. (Wash.) 70, 2642–2646 (1973)
Galloway, S.M., Evans, H.J.: Asymmetrie C-bands and satellite DNA in man. Exp. Cell Res. 94, 454–459 (1975)
Gibson, D.A., Prescott, D.M.: Frequency and sites of sister chromatid exchanges in rat kangaroo chromosomes. Exp. Cell Res. 86, 209–214 (1974)
Hatch, F.T., Mazrimas, J.A.: Fractionation and characterisation of satellite DNAs of the kangaroo rat (Dipodomys ordii). Nucleic Acids Res. 1, 559–575 (1974)
Hoehn, H., Martin, G. M.: Clonal variants of constitutive heterochromatin of human fibroblasts after recovery from Mitomycin treatment. Chromosoma (Berl.) 43, 203–210 (1973)
Hsu, T.C.: Longitudinal differentiation of chromosomes. Ann. Rev. Genet. 7, 153–176 (1973)
John, B., Lewis, K.R.: The meiotic system. Protoplasmatologia VI/F1. Wien-New York: Springer 1965
Latt, S. A.: Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)
Latt, S.A., Davidson, R.L., Lin, M.S., Gerald, P.S.: Lateral asymmetry in the fluorescence of human Y chromosomes stained with 33258 Hoechst. Exp. Cell Res. 87, 425–429 (1974)
Lin, M.S., Latt, S.A., Davidson, R.L.: Microfluorometric detection of asymmetry in the centromeric region of mouse chromosomes. Exp. Cell Res. 86, 392–395 (1974)
Marin, G., Prescott, D. M.: The frequency of sister chromatid exchanges following exposure to varying doses of H3-thymidine or X-rays. J. Cell Biol. 21, 159–167 (1964)
Perry, P., Wolff, S.: New Giemsa method for the differential staining of sister chromatids. Nature (Lond.) 251, 156–158 (1974)
Prescott, D.M., Bostock, C. J., Hatch, F.T., Mazrimas, J.A.: Location of satellite DNAs in the chromosomes of the kangaroo rat (Dipodomys ordii). Chromosoma (Berl.) 42, 205–213 (1973)
Smith, G.: Unequal cross-over and the evolution of multigene families. Cold Spr. Harb. Symp. quant. Biol. 38, 507–513 (1973)
Smith, G.: Evolution of repeated DNA sequences by unequal cross-over. Science 191, 528–535 (1976)
Southern, E.M.: Long range periodicities in mouse satellite DNA. J. molec. Biol. 94, 51–69 (1975)
Sumner, A.T.: A simple technique for demonstrating centromeric heterochromatin. Exp. Cell Res. 75, 304–306 (1972)
Walen, K.H.: Somatic crossing over in relationship to heterochromatin in Drosophila melanogaster. Genetics 49, 905–923 (1964)
Walker, P.M.B., Flamm, W.G., McLaren, A.: Highly repetitive DNA in rodents. In: Handbook of molecular cytology (A. Lima-de-Faria, ed.), pp. 52–66. Amsterdam and London: North Holland Publishing Co. 1969
Wolff, S., Perry, P.: Differential Giemsa staining of sister chromatids and the study of sister chromatid exchanges without autoradiography. Chromosoma (Berl.) 48, 341–353 (1974)
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Bostock, C.J., Christie, S. Analysis of the frequency of sister chromatid exchange in different regions of chromosomes of the kangaroo rat (Dipodomys ordii). Chromosoma 56, 275–287 (1976). https://doi.org/10.1007/BF00293191
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DOI: https://doi.org/10.1007/BF00293191