Skip to main content
SpringerLink
Log in

Isolation from Salmonella typhimurium LT2 of mutants lacking specifically nitrate reductase activity and mapping of the chl-C gene

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Addition of tetrathionate and nitrate to EMB medium allows the isolation from Salmonella typhimurium LT2 of mutants lacking specifically nitrate reductase A activity. Genetic localization of such chl-C mutations leads to the conclusion that the affected gene is close to tdk but outside the trp-tdk segment and that chl-C is an early marker in Hfr B2.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adelberg, E.A., Mandel, M., Chein Ching Chen, G.: Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli K12. Biochem. Biophis. Res. Commun. 18, 788–795 (1965).

    Google Scholar 

  • Beck, C.F., Ingraham, J.L., Neuhard, J.: Location on the chromosome of Salmonella typhimurium of genes governing pyrimidine metabolism. II—Uridine kinase, cytosine deaminase and thymidine kinase. Molec. gen. Genet. 115, 208–215 (1972).

    Google Scholar 

  • Casse, F., Pascal, M.C., Chippaux, M.: A mutant of Salmonella typhimurium deficient in tetrathionate reductase activity. Molec. gen. Genet. 119, 71–74 (1972a).

    Google Scholar 

  • Casse, F., Pascal, M.C., Chippaux, M., Ratouchniak, J.: Mapping of the chl B gene in Salmonella typhimurium LT2. Molec. gen. Genet. 119, 67–70 (1972b).

    Google Scholar 

  • Chippaux, M., Casse, F., Pascal, M.C.: Isolation and phenotypes of mutants from Salmonella typhimurium defective in formate hydrogenlyase activity. J. Bact. 110, 766–768 (1972).

    Google Scholar 

  • Le Minor, L., Chippaux, M., Pichinoty, F., Coynault, C., Piechaud, M.: Méthodes simples premettant de rechercher la tétrathionate-réductase en cultures liquides ou sur colonies isolées. Ann. Inst. Pasteur 119, 733–737 (1970).

    Google Scholar 

  • Pascal, M. C., Casse, F., Chippaux, M., Lepelletier, M.: Genetic analysis of mutants of Salmonella typhimurium deficient in formate dehydrogenase. Molec. gen. Genet. 120, 337–340 (1973).

    Google Scholar 

  • Pichinoty, F., Chippaux, M.: Recherche sur des mutants bactériens ayant perdu les activités catalytiques liées à la nitrate-réductase A. III-Caractères biochimiques. Ann. Inst. Pasteur 117, 145–178 (1969).

    Google Scholar 

  • Piechaud, M., Puig, J., Pichinoty, F., Azoulay, E., Le Minor, L.: Mutations affectant la nitrate-réductase A et d'autres enzymes bactériens d'oxydo-réduction. Etude préliminaire. Ann. Inst. Pasteur 112, 24–37 (1967).

    Google Scholar 

  • Puig, J., Azoulay, E., Pichinoty, F., Gendre, J.: Genetic mapping of the chl C gene of nitrate reductase A system in Escherichia coli K12. Biochem. Biophys. Res. Comm. 35, 659–662 (1969).

    Google Scholar 

  • Saint-Pierre, M.L.: Isolation and mapping of Salmonella typhimurium mutants defective in the utilization of trehalose. J. Bact. 95, 1185–1186 (1968).

    Google Scholar 

  • Stouthamer, A. M.: A genetical and biochemical study of chlorate resistant mutants of Salmonella typhimurium. Antonie van Leeuwenhoek, 35, 505–521 (1969).

    Google Scholar 

  • Taylor, A.L.: Current linkage map of Escherichia coli, Bact. Rev. 34, 155–175 (1970).

    Google Scholar 

  • Tseng-Tong Kuo, Macphee, D. G., Krishnapillai, V., Stocker, B. A. D.: Salmonella thypimurium mutants deficient of UDP glucose pyrophos-phorylase: Genetics and cultural properties. Personnal communication (1972).

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Chimie Bactérienne, C.N.R.S., Marseille, France

    Francine Casse, Marc Chippaux & Marie-Claire Pascal

Authors
  1. Francine Casse
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marc Chippaux
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marie-Claire Pascal
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Gros

Rights and permissions

Reprints and permissions

About this article

Cite this article

Casse, F., Chippaux, M. & Pascal, MC. Isolation from Salmonella typhimurium LT2 of mutants lacking specifically nitrate reductase activity and mapping of the chl-C gene. Molec. Gen. Genet. 124, 247–251 (1973). https://doi.org/10.1007/BF00293095

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00293095

Keywords

Search

Navigation