Abstract
An antigen making a major contribution to the immune response to Drosophila melanogaster chromatin resides primarily on a nonhistone charge-class family of proteins of Mr 74,000. Immunofluorescence detects this antigen at interbands, puffs and diffuse bands of D. melanogaster salivary chromosomes isolated without exposure to acid fixatives, and on nucleoplasmic ribonucleoprotein droplets. In the electron microscope, gold labelling reveals the binding of monoclonal antibodies specific for the antigen at chromosomal loci generally bearing putative ribonucleoprotein (RNP) particles. However, the locus 3C 11–12 is remarkable in that it bears putative RNP particles but is virtually unlabelled, suggesting protein specificity at different active loci.
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Hill, R.J., Mott, M.R., Watt, F. et al. The localisation of an Mr 74,000 major chromatin antigen on native salivary chromosomes of Drosophila melanogaster . Chromosoma 94, 441–448 (1986). https://doi.org/10.1007/BF00292753
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DOI: https://doi.org/10.1007/BF00292753