Abstract
Fixed metaphase chromosomes from mouse strain RIII embryos or A9 cells were treated with a restriction endonuclease, followed by Giemsa staining. Aha I, Hinf I, or Mbo I treatment produced a C-band pattern, and Eco RII or Hae III produced a G-band plus C-band pattern. Ava II and Bst NI each produced a G-band pattern, but on most chromosomes only a small segment of each C-band, adjacent to the centromere, was stained. These tiny residual C-bands may contain a minor satellite located adjacent to the major satellite clusters.
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Kaelbling, M., Miller, D.A. & Miller, O.J. Restriction enzyme banding of mouse metaphase chromosomes. Chromosoma 90, 128–132 (1984). https://doi.org/10.1007/BF00292449
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DOI: https://doi.org/10.1007/BF00292449