Abstract
Chinese hamster ovary cells (CHO cells) and mouse fibroblasts (PG 19) were permeabilized with inactivated Sendai virus, treated with different types of restriction endonucleases (Eco RV, Pvu II, Bam HI, Sma I, Asu III, Nun II), and studied for the occurrence of chromosomal aberrations at different times following treatment. The pattern of chromosomal aberrations observed was similar to that induced by ionizing radiations. Restriction endonucleases that induce blunt double-strand breaks (Eco RV, Pvu II) were more efficient in inducing chromosomal aberrations than those that induce breaks with cohesive ends (Bam HI, Nun II, Asu III). Ring types were very frequent among the aberrations induced by restriction enzymes. Cytosine arabinoside, an inhibitor of DNA repair, was found to increase the frequencies of aberrations induced by restriction enzymes, indicating its effect on ligation of double-strand breaks. The relevance of these results to the understanding of the mechanisms of chromosomal aberration formation following treatment with ionizing radiations is discussed.
Similar content being viewed by others
References
Bender MA, Griggs HG, Bedford JS (1974) Mechanisms of chromosomal aberration production. III. Chemicals and ionizing radiations, Mutat Res 23:197–212
Enzymatic Restriction of in situ mammalian cell DNA using Pvu II and Bam H1: evidence for the double strand break origin of chromosomal aberrations. Int J Radiat Biol (in press)
Evans HJ, Savage JRK (1963) The relation between DNA synthesis and chromosome structure as revealed by X-ray damage. J Cell Biol 18:535–540
Kihlman BA, Natarajan AT (1984) Potentiation of chromosomal alterations by inhibitors of DNA repair, in: Nucleic Acid Research (in press)
Luchnik NV, Fesenko EV, Orchinnikova VG (1976) Critical periods of the mitotic cycle: influence of aminopterin and thymidine on production of chromosomal aberrations by radiations in Crepsis capillaris, Mutat Res:34, 367–388
Natarajan AT (1983) Mutagenesis and chromosome changes — review, In: Broerse JJ, Barendsen GW, Kal HB, van der Kogel AJ (eds) Radiation research, proceedings of the 7th International Congress of Radiation Research, pp 239–247
Natarajan AT, Obe G (1978) Molecular mechanisms involved in the production of chromosomal aberrations. I. Utilization of Neurospora endonuclease for the study of aberration production in G2 state of the cell cycle, Mutat Res 52:137–149
Natarajan AT, Obe G, van Zeeland AA, Palitti F, Meijers M, Verdegaal-Immerzeel EAM (1980) Molecular mechanisms involved in the production of chromosomal aberrations. II. Utilization of neurospora endonuclease for the study of aberration production by X rays in G1 and G2 stages of the cell cycle, Mutat Res 69:293–305
Natarajan AT, Obe G, Altmann H (1982) Preface in DNA repair, chromosome alerations and chromatin structure, Elsevier Biomedical Press V-IX
Nowak C, Obe G, van Kesteren-van Leeuwen AC, Natarajan AT (1984) A cytological test system to detect mutagen-induced single-strand breaks: post-treatment of G2 phase CHO cells with Neurospora endonuclease, Mutat Res (in press)
Obe G, Natarajan AT, Palitti F (1982) Role of DNA double-strand breaks in the formation of radiation-induced chromosomal aberrations, in: Natarajan AT, Obe G, Altmann H (eds) DNA repair, chromosome alterations and chromatin structure. Elsevier Biomedical Press, pp 1–9
Preston RJ (1982) DNA repair and chromosome aberrations: interactive effects of radiation and chemicals, in: Natarajan AT, Obe G, Altmann H (eds) DNA repair, chromosome alterations and chromatin structure. Elsevier Biomedical Press, pp 25–35
Sax K (1941) Types and frequencies of chromosomal aberrations induced by X rays, Cold Spring Harbor Symp Quant Biol:93–103
Smith CA (1984) Analysis of repair synthesis in the presence of inhibitors, Nucleic Acid Research (in press)
Wolff S, Luipold HE (1964) Chromosome splitting as revealed by X-ray and labelling experiments. Exptl Cell Res 55:9–22
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Natarajan, A.T., Obe, G. Molecular mechanisms involved in the production of chromosomal aberrations. Chromosoma 90, 120–127 (1984). https://doi.org/10.1007/BF00292448
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00292448