Summary
NADH-cytochrome b5 reductase (DIA1, EC. 1.6.2.2) from human fibroblasts and from Chinese hamster cells, both identified by immunologic studies, were clearly distinguished after polyacrylamide gel isoelectrofocusing followed by staining for NADH diaphorase activity. In thirteen independent man-hamster hybrids, the human enzyme DIA1 presented a positive correlation with the human chromosome G22. Eight hybrids were DIA1(+) G22(+) and five hybrids were DIA1(-) G22(-). These data agree with the recent assignment of DIA1 to chromosome G22 by Fisher et al. (1977a). We assume that this newly assigned locus codes for both soluble and microsomal forms of NADH-cytochrome b5 reductase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Davidson, R. L., Gerald, P. S.: Improved techniques for the induction of mammalian cell hybridization by polyethylene glycol. Somat. Cell. Gen. 2, 165–176 (1976)
Drysdale, J. W., Righetti, P., Bunn, H. F.: The separation of human and animal hemoglobins by isoelectric focusing in polyacrylamide gel. Biochim. Biophys. Acta 229, 42–50 (1970)
Dutrillaux, B., Lejeune, J.: Sur une nouvelle technique d'analyse du caryotype humain. C.R. Acad. Sci. (Paris) 272, 2638–2640 (1971)
Fisher, R. A., Edwards, Y. H., Putt, W., Potter, J., Hopkinson, D. A.: An interpretation of human diaphorase isozymes in terms of three gene loci: DIA1, DIA2, and DIA3. Ann. Hum. Genet. 41, 139–149 (1977a)
Fisher, R. A., Povey, S., Bobrow, M., Solomon, E., Boyd, Y., Carritt, B.: Assignment of the DIA1 locus to chromosome 22. Ann. Hum. Genet. 41, 151–155 (1977b)
Goto-Tamura, R., Takesue, Y., Takesue, S.: Immunological similarity between NADH-cytochrome b5 reductase of erythrocytes and liver microsomes. Biochim. Biophys. Acta 423, 293–302 (1976)
Hegesh, E., Calmanovici, N., Avron, M.: New method for determining ferrihemoglobin reductase (NADH-methemoglobin reductase) in erythrocytes. J. Lab. Clin. Med. 72, 339–344 (1968)
Kaplan, J. C., Beutler, E.: Electrophoresis of red-cell NADH and NADPH-diaphorases in normal subjects and patients with congenital methemoglobinemia. Biochem. Biophys. Res. Commun. 29, 605–610 (1967)
Kaplan, J. C., Leroux, A., Beauvais, P.: Clinical and biological heterogeneity of NADH-cytochrome b5 reductase deficiency. Hum. Hered. 27, 188 (1977)
Klebe, R. J., Tchaw-ren Chen, Ruddle, F. H.: Controlled production of proliferating somatic cell hybrids. J. Cell Biol. 45, 74–82 (1970)
Kuma, F., Prough, R. A., Masters, B. S. S.: Studies on methemoglobin reductase. Immunochemical similarity of soluble methemoglobin reductase and cytochrome b5 of human erythrocytes with NADH-cytochrome b5 reductase and cytochrome b5 of rat liver microsomes. Arch. Biochem. Biophys. 172, 600–607 (1976)
Leroux, A., Kaplan, J. C.: Presence of red-cell type NADH-methemoglobin reductase (NADH-diaphorase) in human nonerythroid cells. Biochem. Biophys. Res. Commun. 49, 945–950 (1972)
Leroux, A., Junien, C., Kaplan, J. C., Bamberger, J.: Generalised deficiency of cytochrome b5 reductase in congenital methaemoglobinaemia with mental retardation. Nature 258, 619–620 (1975)
Leroux, A., Torlinski, L., Kaplan, J. C.: Soluble and microsomal forms of NADH-cytochrome b5 reductase from human placenta. Similarity with NADH-methemoglobin reductase from human erythrocytes. Biochim. Biophys. Acta 481, 50–62 (1977)
Littlefield, J. W.: Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145, 709–710 (1964)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Passon, P. G., Hultquist, D. E.: Soluble cytochrome b5 reductase from human erythrocytes. Biochim. Biophys. Acta 275, 62–73 (1972)
Pontecorvo, G.: Production of mammalian somatic cell hybrids by means of polyethylene glycol treatment. Somat. Cell Genet. 1, 397–400 (1975)
Strittmatter, P.: Microsomal cytochrome b5 and cytochrome b5 reductase. In: The enzymes, P. D. Boyer, M. Lardy, K. Myrbäck, eds., Vol. 8, pp. 113–145. New York: Academic Press 1963
Author information
Authors and Affiliations
Additional information
Groupe INSERM U 129: Directeur J. C. Dreyfus
Groupe INSERM U 129: Directeur J. C. Dreyfus
Rights and permissions
About this article
Cite this article
Junien, C., Vibert, M., Weil, D. et al. Assignment of NADH-cytochrome b5 reductase (DIA1 locus) to human chromosome 22. Hum Genet 42, 233–239 (1978). https://doi.org/10.1007/BF00291301
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00291301