Summary
The gametophytic self-incompatibility system of Solarium tuberosum is controlled by a single locus, designated as the S-locus. Protein extracts from potato styles of defined S-genotypes have been analysed by two-dimensional gel electrophoresis, and found to contain a group of basic glycoproteins. Each genetically determined allele S 1 to S 4 was associated with the presence of one of a number of these polypeptides differing slightly in isoelectric points (in the range 8.3–>9.1) and/or apparent molecular weight (ranging from 23,000 to 29,000). Two abundant basic polypeptides, one of which is apparently not glycosylated, were present in all genotypes examined. Amino-terminal protein sequence determinations revealed homologies of the S. tuberosum stylar proteins S2, S3 and S4 with SI-associated polypeptides from Nicotiana alata and Lycopersicon peruvianum. With an oligonucleotide generated to the potato-S2 N-terminal protein sequence, it was possible to detect a style-specific RNA species of ∼920 nucleotides. The oligonucleotide also behaved as an allele-specific probe when hybridized to total RNA of different S-genotypes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ames G, Nikaido K (1976) Two-dimensional gel electrophoresis of membrane proteins. Biochemistry 15:616–623
Anderson MA, Cornish EC, Mau SL, Williams EG, Hoggart R, Atkinson A, Bonig I, Grego B, Simpson R, Roche PJ, Haley JD, Penschow JD, Niall HD, Tregear GW, Coghlan JP, Crawford RJ, Clarke AE (1986) Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata. Nature 321:38–44
Beaucage SL, Caruthers MH (1981) Deoxynucleoside phosphoramidites: a new class of key intermediates for deoxypolynucleotide synthesis. Tetrahedron Lett 22:1859–1862
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Bredemeijer GMM, Blaas J (1981) S-specific proteins in styles of self-incompatible Nicotiana alata. Theor Appl Genet 59:185–190
Cipar MS, Peloquin SJ, Hougas RW (1964) Inheritance of incompatibility in hybrids between Solanum tuberosum haploids and diploid species. Euphytica 13:163–172
Crane MB, Lawrence WJC (1929) Genetical and cytological aspects of incompatibility and sterility in cultivated fruits. J Pomol Hortic Sci 7:276–301
De Nettancourt D (1977) Incompatibility in angiosperms. (Monographs on theoretical and applied genetics, vol 3). Springer, Berlin Heidelberg New York
Eckerskorn C, Mewes W, Goretzki H, Lottspeich F (1988) A new siliconized-glass fiber as support for protein-chemical analysis of electroblotted proteins. Eur J Biochem 176:509–519
Hawkes R (1982) Identification of Con A-binding proteins after SDS-gel electrophoresis and protein blotting. Anal Biochem 123:143–146
Hinata K, Nishio T (1978) S-allele specificity of stigma proteins in Brassica oleracea and Brassica campestris. Heredity 41:93–100
Hurkman WJ, Tanaka CK (1986) Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol 81:802–806
Kamboj RK, Jackson JF (1986) Self-incompatibility alleles control a low molecular weight, basic protein in pistils of Petunia hybrida. Theor Appl Genet 71:815–819
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:681–685
Logemann J, Schell J, Willmitzer L (1987) Improved method for the isolation of RNA from plant tissues. Anal Biochem 163:16–20
Lottspeich F (1985) Microscale isocratic separation of phenylthiohydantoin amino acid derivatives. J Chromatogr 326:321–324
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory Press, Cold Spring Harbour/NY
Mau SL, Williams EG, Atkinson A, Anderson MA, Cornish EC, Grego B, Simpson RJ, Kheyr-Pour A, Clarke AE (1986) Style proteins of a wild tomato (Lycopersicon peruvianum) associated with expression of self-incompatibility. Planta 169:184–191
Morrissey JH (1981) Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity. Anal Biochem 117:307–310
Nasrallah JB, Nasrallah ME (1984) Electrophoretic heterogeneity exhibited by the S-allele specific glycoproteins of Brassica. Experientia 40:279–281
O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021
O'Farrell PZ, Goodman HM, O'Farrell PH (1977) High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12:1133–1142
Pandey KK (1962 a) Interspecific incompatibility in Solanum species. Am J Bot 49:874–882
Pandey KK (1962 b) Incompatibility in some Solanum crosses. Genetica 33:24–30
Raff JW, Knox RB, Clarke AE (1981) Style antigens of Prunus avium L. Planta 153:125–129
Sanchez-Martinez D, Puigdomenech P, Pages M (1986) Regulation of gene expression in developing Zea mays embryos. Plant Physiol 82:543–549
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Woods DE (1984) Oligonucleotide screening of cDNA libraries. BRL Focus 6, No 3:1–2
Author information
Authors and Affiliations
Additional information
Communicated by H. F. Linskens
Rights and permissions
About this article
Cite this article
Kirch, H.H., Uhrig, H., Lottspeich, F. et al. Characterization of proteins associated with self-incompatibility in Solanum tuberosum . Theoret. Appl. Genetics 78, 581–588 (1989). https://doi.org/10.1007/BF00290845
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00290845