Skip to main content
Log in

Genetic variability for heat shock proteins in common wheat

  • Originals
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

The response of the common wheat line ‘Chinese Spring’ to heat shocks of different time lengths was studied by the two-dimensional (2D) electrophoresis of denatured proteins. After a heat shock of 5 h, 33 heat shock proteins (HSPs) accumulated in an amount sufficient to be revealed by silver stain. Two other wheat lines (‘Moisson’ and ‘Selkirk’) were then submitted to a heat shock of 5 h, and the responses of the 3 lines were compared: of a total of 35 HSPs, 13 (37.1%) were quantitatively or qualitatively variable. This variability concerns low-molecular-weight and high-molecular-weight HSPs. The three genotypes showed thermal tolerance but ‘Chinese Spring's’ response to heat treatments was slightly different from those of the other two lines The possibility of a relationship between HSP patterns and thermal sensitivity is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Colas des Francs C, Thiellement H (1985) Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines. Theor Appl Genet 71:31–38

    Google Scholar 

  • Cooper P, Ho T-HD, Hauptmann R-M (1984) Tissue specificity of the heat-shock response in maize. Plant Physiol 75:431–441

    Google Scholar 

  • Damerval C, de Vienne D, Zivy M, Thiellement H (1986) Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheatseedling proteins. Electrophoresis 7:52–54

    Google Scholar 

  • Granier F, de Vienne D (1986) Silver staining of proteins: standardized procedure for two-dimensional gels bound to polyester sheets. Anal Biochem 155:45–50

    Google Scholar 

  • Kee SC, Nobel PS (1986) Concomitant changes in high temperature tolerance and heat-shock proteins in desert succulents. Plant Physiol 80:596–598

    Google Scholar 

  • Kelley P, Schlesinger M (1982) Antibodies to two major heat shock proteins cross-react with similar proteins in widely divergent species. Mol Cell Biol 2:267–274

    Google Scholar 

  • Key JL, Kimpel JA, Lin ChY, Nagao RT, Vierling E, Czarnecka E, Gurley WB, Roberts JK, Mansfield MA, Edelman L (1985) The heat shock response in soybean. In: Key JL, Kosuge T (eds) Cellular and molecular biology of plant stress. Alan R Liss, New York, pp 161–179

    Google Scholar 

  • Kimpel JA, Key JL (1985) Heat shock in plants. Trends Biochem Sci 85:353–357

    Google Scholar 

  • Lin Cy, Roberts JK, Key JL (1984) Acquisition of thermotolerance in soybean seedlings. Synthesis and accumulation of heat shock proteins and their cellular localization. Plant Physiol 74:152–160

    Google Scholar 

  • Loomis WF, Wheeler SA (1982) Chromatine-associated heatshock proteins of Dictyostelium. Dev Biol 90:412–418

    Google Scholar 

  • Lopato SV, Gleba YY (1985) Heat shock proteins from cell cultures of higher plants and their somatic hybrids. Plant Cell Rep 4:19–22

    Google Scholar 

  • McAlister L, Finkelstein DB (1980) Heat shock proteins and thermal resistance in yeast. Biochem Biophys Res Commun 93:819–824

    Google Scholar 

  • Moran LA, Chauvin M, Kennedy ME, Korri M, Lowe DG, Nicholson RC, Perry MD (1983) The major heat shock protein (hsp70) gene family: related sequences in mouse, Drosophila, and yeast. Can J Biochem Cell Biol 61:488–499

    Google Scholar 

  • Oakley BR, Kirsh DR, Morris NR (1980) A simplified silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 105:361–363

    Google Scholar 

  • O'Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    Google Scholar 

  • Schlesinger MJ, Ashburner M, Tissieres A (1982) Heat shock: from bacteria to man. Cold Spring Harbor, New York

  • Velasquez JM, DiDomenico BJ (1980) Intracellular localization of heat shock proteins in Drosophila. Cell 20:679–689

    Google Scholar 

  • Vierling E, Mishkind ML, Schmidt GW, Key JL (1986) Specific heat shock proteins are transported into chloroplasts. Proc Natl Acad Sci USA 83:361–365

    Google Scholar 

  • Yamamori T, Yura T (1982) Genetic control of heat shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12. Proc Natl Acad Sci USA 79:860–864

    Google Scholar 

  • Zannis VI, Breslow JL (1981) Human very low density apolipoprotein E isoprotein polymorphism is explained by genetic variation and posttranslational modification. Biochemistry 20:1033–1041

    Google Scholar 

  • Zimmermann JL, Petri W, Meselson M (1983) Accumulation of a specific subset of D. melanogaster heat shock mRNA's in normal development without heat shock. Cell 32:1161–1170

    Google Scholar 

  • Zivy M, Thiellement H, de Vienne D, Hofmann J-P (1984) Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis. 2. Genetic differences between two lines and two groups of cytoplasms at five developmental stages or organs. Theor Appl Genet 68:335–345

    Google Scholar 

  • Zivy M (1986) Influence des ampholytes sur la révélation au nitrate d'argent. In: Galteau M-M, Siest G (eds) Recent progresses in two-dimensional electrophoresis. Press University Nancy, pp 69–72

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by H. F. Linskens

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zivy, M. Genetic variability for heat shock proteins in common wheat. Theoret. Appl. Genetics 74, 209–213 (1987). https://doi.org/10.1007/BF00289970

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00289970

Key words

Navigation