Summary
The effect of liquid holding treatment after UV exposure has been determined in diploid yeast cultures carrying genes for UV sensitivity in both the homozygous and heterozygous condition. In all cultures liquid holding treatment produced increases in cell viability, but in cultures carrying a UVS gene this was not correlated with the increase in the frequency of intragenic recombinants characteristic of cultures wild type for UV sensitivity, but with a significant decrease in recombinant frequency. The data presented indicates that the reduction in recombinant frequency was not correlated with a reduction in the total number of recombinants after liquid holding treatment but was due to a selective increase in the frequency of non-recombinant cells.
Similar content being viewed by others
References
Cox, B. S., Bevan, E. A.: Aneuploidy in yeast. New Phytol. 61, 342 (1962).
Ganeson, A. K., Smith, K. C.: Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec− mutations on liquid holding recovery. J. Bact. 96, 365–373 (1968).
—: II. Effect of uvr genes on liquid holding recovery. J. Bact. 97, 1129–1133 (1969).
Hanawalt, P. C.: Cellular recovery from photochemical damage. In: Photophysiology, col. IV, p. 203–251 (ed. Giese, A. C.). New York: Academic Press 1968.
Haynes, R. H., Baker, R. M., Jones, G. E.: Genetic implications of DNA repair. In: Energetics and mechanisms in radiation biology, p. 425–465 (ed. Phillips, G.). New York: Academic Press 1968.
Howard-Flanders, P.: DNA repair. Ann. Rev. Biochem. 37, 175–200 (1968).
Mortimer, R. K., Hawthorne, D. C.: Genetic mapping in Saccharomyces. Genetics 63, 165 (1963).
Parry, J. M., Cox, B. S.: Photoreactivation of UV induced reciprocal recombination, gene conversion and mutation to prototrophy in Saccharomyces cerevisiae. J. gen. Microbiol. 40, 235–241 (1965).
— The effects of dark holding and photoreactivation on ultraviolet lightinduced mitotic recombination and survival in yeast. Genet. Res. 12, 187 (1968).
— Parry, E. M.: The effects of UV-light post-treatment on the survival characteristics of 21 UV-sensitive mutants of Saccharomyces cerevisiae. Mutation Res. 8, 545–556 (1969).
Parry, J. M. The genetic implications of UV light exposure and liquid-holding posttreatment in the yeast Saccharomyces cerevisiae. (1971), in preparation.
Setlow, J.: Photoreactivation. Radiat. Res., Suppl. 6, 141–155 (1966).
Setlow, R. B.: The photochemistry, the photobiology and repair of polynucleotides. Progr. Nucleic Acid. Res. Mol. Biol. 8, 257–295 (1968).
Strauss, B.: DNA repair mechanisms and their relation to mutation and recombination. Current topics in Microbiol. Immunol. 44, 1–85 (1968).
Witkin, E.: UV induced mutation and DNA repair. Ann. Rev. Genet. 3, 525–552 (1969).
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Parry, J.M. The genetic effects of liquid holding recovery in ultra violet light sensitive mutants of yeast. Molec. Gen. Genet. 111, 51–60 (1971). https://doi.org/10.1007/BF00286553
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00286553