Skip to main content
Log in

The genetic effects of liquid holding recovery in ultra violet light sensitive mutants of yeast

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The effect of liquid holding treatment after UV exposure has been determined in diploid yeast cultures carrying genes for UV sensitivity in both the homozygous and heterozygous condition. In all cultures liquid holding treatment produced increases in cell viability, but in cultures carrying a UVS gene this was not correlated with the increase in the frequency of intragenic recombinants characteristic of cultures wild type for UV sensitivity, but with a significant decrease in recombinant frequency. The data presented indicates that the reduction in recombinant frequency was not correlated with a reduction in the total number of recombinants after liquid holding treatment but was due to a selective increase in the frequency of non-recombinant cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Cox, B. S., Bevan, E. A.: Aneuploidy in yeast. New Phytol. 61, 342 (1962).

    Google Scholar 

  • Ganeson, A. K., Smith, K. C.: Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec mutations on liquid holding recovery. J. Bact. 96, 365–373 (1968).

    Google Scholar 

  • —: II. Effect of uvr genes on liquid holding recovery. J. Bact. 97, 1129–1133 (1969).

    Google Scholar 

  • Hanawalt, P. C.: Cellular recovery from photochemical damage. In: Photophysiology, col. IV, p. 203–251 (ed. Giese, A. C.). New York: Academic Press 1968.

    Google Scholar 

  • Haynes, R. H., Baker, R. M., Jones, G. E.: Genetic implications of DNA repair. In: Energetics and mechanisms in radiation biology, p. 425–465 (ed. Phillips, G.). New York: Academic Press 1968.

    Google Scholar 

  • Howard-Flanders, P.: DNA repair. Ann. Rev. Biochem. 37, 175–200 (1968).

    Google Scholar 

  • Mortimer, R. K., Hawthorne, D. C.: Genetic mapping in Saccharomyces. Genetics 63, 165 (1963).

    Google Scholar 

  • Parry, J. M., Cox, B. S.: Photoreactivation of UV induced reciprocal recombination, gene conversion and mutation to prototrophy in Saccharomyces cerevisiae. J. gen. Microbiol. 40, 235–241 (1965).

    Google Scholar 

  • — The effects of dark holding and photoreactivation on ultraviolet lightinduced mitotic recombination and survival in yeast. Genet. Res. 12, 187 (1968).

    Google Scholar 

  • — Parry, E. M.: The effects of UV-light post-treatment on the survival characteristics of 21 UV-sensitive mutants of Saccharomyces cerevisiae. Mutation Res. 8, 545–556 (1969).

    Google Scholar 

  • Parry, J. M. The genetic implications of UV light exposure and liquid-holding posttreatment in the yeast Saccharomyces cerevisiae. (1971), in preparation.

  • Setlow, J.: Photoreactivation. Radiat. Res., Suppl. 6, 141–155 (1966).

    Google Scholar 

  • Setlow, R. B.: The photochemistry, the photobiology and repair of polynucleotides. Progr. Nucleic Acid. Res. Mol. Biol. 8, 257–295 (1968).

    Google Scholar 

  • Strauss, B.: DNA repair mechanisms and their relation to mutation and recombination. Current topics in Microbiol. Immunol. 44, 1–85 (1968).

    Google Scholar 

  • Witkin, E.: UV induced mutation and DNA repair. Ann. Rev. Genet. 3, 525–552 (1969).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by F. Kaudewitz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Parry, J.M. The genetic effects of liquid holding recovery in ultra violet light sensitive mutants of yeast. Molec. Gen. Genet. 111, 51–60 (1971). https://doi.org/10.1007/BF00286553

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00286553

Keywords

Navigation