Abstract
RNA synthesis following 3H-uridine pulse labeling has been studied autoradiographically in the 1B-3C region of D. melanogaster salivary gland X-chromosome. To improve the precision of RNA synthesis site localization in bands and interbands a micromanipulator technique of chromosome stretching was used. No significant RNA synthesis was detected in the interbands. RNA synthesis was found to occur not only in puffs but also in bands whose puffing was not apparent. In 5 genetically studied segments of the 1B-3C region where 56 complementation groups have been identified (Lefevre, 1974; Rayle, 1972; Gvozdev et al., 1975; Judd et al., 1974; Lefevre and Green, 1972), at least 21 transcription sites are detected.
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References
Ananiev, E.V., Barsky, V.E.: DNA content, replication and transcription in different sites of the X-chromosome of Drosophila melanogaster. J. molec. Biol. (Russ.) 9, 752–760 (1975)
Ashburner, M.: Structure and function of polytene chromosomes during insect development. Advanc. Insect Physiol. 7, 1–95 (1970)
Beermann, W.: Riesenchromosomen. Protoplasmatologia. VI/D. Wien: Springer 1962
Beermann, W.: Developmental studies on giant chromosomes. In: Results and problems in cell differentiation Berlin-Heidelberg-New York: Springer 1972
Beermann, W., Clever, U.: Chromosome puffs. Sci. Amer. 210, 50–58 (1964)
Berendes, H.D.: Salivary gland function and chromosomal puffing patterns in Drosophila hydei. Chromosoma (Berl.) 17, 35–77 (1966)
Berendes, H.D.: Polytene chromosome structure at the submicroscopic level. Chromosoma (Berl.) 29, 118–130 (1970)
Bridges, C.B.: Revised salivary map of X-chromosomes of Drosophila melanogaster. J. Hered. 29, 11–13 (1938)
Crick, F.: General model for the chromosomes of higher organisms. Nature (Lond.) 234, 25–27 (1971)
Edström, I.E., Beermann, W.: The base composition of nucleic acid in chromosomes, puffs, nucleoli and cytoplasm of Chironomus salivary gland cells. J. Cell. Biol. 14, 371–378 (1962)
Ephrussi, B., Beadle, G.B.: Technique of transplantation for Drosophila. Amer. Naturalist 70, 218–225 (1936)
Fujita, S., Takomoto, K.: Synthesis of messenger RNA on the polytene chromosomes of dipteran salivary gland. Nature (Lond.) 200, 494–497 (1963)
Gall, J.G., Callan, H.G.: H3-uridine incorporation in lampbrush chromosomes. Proc. nat. Acad. Sci. (Wash.) 48, 562–570 (1962)
Gvozdev, V.A., Gostimsky, S.A., Gerasimova, I.I., Dubrovskaya, Ye.S., Braslavskaya, O.Yu.: Fine structure analysis at the 2D3-2F5 region of the X-chromosome of Drosophila melanogaster. Molec. gen. Genet. 141, 269–275 (1975)
Judd, B.H., Young, M.W.: An examination of the one cistron: one chromomere concept. Cold Spr. Harb. Symp. quant. Biol. 38, 573–579 (1974)
Howard, E.F., Plaut, W.: Chromosomal DNA synthesis in Drosophila melanogaster. J. Cell. Biol. 39, 415–429 (1968)
Keyl, H.G., Pelling, C.: Differentielle DNS replication in den Speicheldrüsen-Chromosomen von Chironomus thummi. Chromosoma (Berl.) 14, 347–359 (1963)
Kiknadze, I.I.: Functional organization of chromosomes. Leningrad: Nauka 1972
Lefevre, G.: The relationship between genes and polytene chromosome bands. Ann. Rev. Genet. 9, 51–62 (1974)
Lefevre, G.: A photographs representation and interpretation of the polytene chromosomes of Drosophila melanogaster. In: The genetics and biology of Drosophila (M. Ashburner and E. Novitski eds), vol. 1a, pp. 29–66. London: Academic Press 1976
Lefevre, G., Green, M.M.: Genetic duplication in the white-split interval of the X-chromosome in Drosophila melanogaster. Chromosoma (Berl.) 36, 391–412 (1972)
Levy, B.W., McCarthy, B.J.: Messenger RNA complexity in Drosohila melanogaster. Biochemistry 14, 2440–2446 (1975)
Lyndsley, D.L., Grell, E.H.: Genetic variations of Drosophila melanogaster. Carnegie Inst. Wash. Publ. 627, 1968
Pelling, C.: Ribonukleinsäure-Synthese der Riesen-Chromosomen. Autoradiographische Untersuchungen an Chrinomus tentans. Chromosoma (Berl.) 15, 71–122 (1964)
Pelling, C.: A replicative and synthetic chromosomal unit — the modern concept of the chromomere. Proc. roy. Soc. (Lond.) B 164, 279–289 (1966)
Pelling, C.: Synthesis of nucleic acids in giant chromosomes. In: Progress in biophysics. vol. 19, pp. 237–270. New York: Pergamon Press 1969
Rayle, R.E.: Genetic analysis of a short X-chromosomal region in D. melanogaster. Genetics 71 (abs) p. 2, 50 (1972)
Swift, H.: Nucleic acids and cell metabolism in dipteran salivary glands. In: The molecular control of cellular activity, p. 73–125. New York: McGraw-Hill Book Co. 1962
Zhimulev, I.F., Belyaeva, E.S.: Proposals to the problem of structural and functional organization of polytene chromosomes. Theoret. Appl. Genet. 45, 335–340 (1975a)
Zhimulev, I.F., Belyaeva, E.S.: H3-uridine labelling patterns in the Drosophila melanogaster salivary gland chromosomes X, 2R and 3L. Chromosoma (Berl.) 49, 219–231 (1975b)
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Ananiev, E.V., Barsky, V.E. Localization of RNA synthesis sites in the 1B-3C region of the Drosophila melanogaster X Chromosome. Chromosoma 65, 359–371 (1978). https://doi.org/10.1007/BF00286415
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DOI: https://doi.org/10.1007/BF00286415