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Studies in heterochromatin DNA: Characterization of transcripts synthesized in situ from C-banded preparations

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Abstract

The transcript of in situ RNA synthesis using Peromyscus eremicus cells, which had been treated with HCl followed by NaOH, proved to be rich in heterochromatin specifying sequences. Such transcript, referred to as heterochromatin RNA, can be characterized biochemically to probe the organization of heterochromatin DNA. It was found that the heterochromatin RNA of P. eremicus contained non-repetitive sequences covalently linked to repetitive sequences.

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Authors and Affiliations

  1. Department of Biology, The University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute, 77030, Houston, Texas, USA

    M. Tien Kuo & T. C. Hsu

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  1. M. Tien Kuo
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  2. T. C. Hsu
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Kuo, M.T., Hsu, T.C. Studies in heterochromatin DNA: Characterization of transcripts synthesized in situ from C-banded preparations. Chromosoma 65, 325–334 (1978). https://doi.org/10.1007/BF00286412

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  • DOI: https://doi.org/10.1007/BF00286412

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