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In situ binding of AT-rich repetitive DNA to the centromeric heterochromatin in polytene chromosomes of chironomids

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Abstract

Native highly repetitive DNA sequences have been allowed to react in situ with DNA-depleted polytene chromosomes of chironomids in cytological preparations. The double-stranded DNA can bind specifically to the centromeric heterochromatin, where these sequences have been localized previously by in situ hybridization. Various control experiments support the conception that heterochromatin-specific DNA-binding proteins are involved in the in situ binding.

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Authors and Affiliations

  1. Lehrstuhl für Genetik der Ruhr-Universität, Postfach 102148, D-4360, Bochum 1, Federal Republic of Germany

    E. R. Schmidt & H. -G. Keyl

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  1. E. R. Schmidt
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  2. H. -G. Keyl
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Dedicated to Prof. Dr. Wolfgang Beermann for his 60th birthday

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Schmidt, E.R., Keyl, H.G. In situ binding of AT-rich repetitive DNA to the centromeric heterochromatin in polytene chromosomes of chironomids. Chromosoma 82, 197–204 (1981). https://doi.org/10.1007/BF00286104

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  • DOI: https://doi.org/10.1007/BF00286104

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