Skip to main content
Log in

Pyridoxine induced puffing (II-48 C) and synthesis of a 40 KD protein in Drosophila hydei salivary glands

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

A specific heat shock puff (II-48 C) has been induced in isolated salivary glands of D. hydei by pyridoxine (vitamin B6) at concentrations from 10−7 to 10−2 M. The puff begins to form within 5 min and continues to increase in size up to 2 h. Under conditions of puff induction the α-amanitin sensitive synthesis of a 40 KD protein is induced.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ashburner, M.: Patterns of puffing activity in salivary gland chromosomes of Drosophila. V. Responses to environmental treatment. Chromosoma (Berl.) 31, 356–376 (1970)

    Google Scholar 

  • Ashburner, M., Bonner, J.J.: The induction of gene activity in Drosophila by heat shock. Cell 17, 241–254 (1979)

    Google Scholar 

  • Berendes, H.D., Breugel, F.M.A. van, Holt, Th.K.H.: Experimental puffs in salivary gland chromosomes of D. hydei. Chromosoma (Berl.) 16, 35–46 (1965)

    Google Scholar 

  • Bisseling, T., Berendes, H.D., Lubsen, N.H.: RNA synthesis in puff 2–48 C after experimental induction in Drosophila hydei. Cell 8, 299–304 (1976)

    Google Scholar 

  • Bonner, J.J., Pardue, M.L.: The effect of heat shock on RNA synthesis in Drosophila tissues. Cell 8, 43–59 (1976)

    Google Scholar 

  • Breugel, F.M.A. van: Puff induction in larval salivary gland chromosomes of D, hydei. Genetica ('s-Gravenhage) 37, 17–28 (1966)

    Google Scholar 

  • Derksen, J., Berendes, H.D., Willart, E.: Production and release of a locus specific ribonucleoprotein product in polytene nuclei of Drosophila hydei. J. Cell Biol. 59, 661–668 (1973)

    Google Scholar 

  • Ellgaard, E.G., Clever, U.: RNA metabolism during puff induction in Drosophila melanogaster. Chromosoma (Berl.) 36, 60–78 (1971)

    Google Scholar 

  • Greenleaf, A.L., Plagens, U., Jamrich, M., Bautz, E.K.F.: RNA polymerase B (or II) in heat-induced puffs on Drosophila polytene chromosomes. Chromosoma (Berl.) 65, 127–136 (1978)

    Google Scholar 

  • Koninkx, J.F.J.G.: Protein synthesis in salivary glands of Drosophila hydei after experimental gene induction. Biochem. J. 158, 623–628 (1976)

    Google Scholar 

  • Leenders, H.J., Berendes, H.D.: The effect of changes in the respiratory metabolism upon genome activity in Drosophila. I. The Induction of gene activity. Chromosoma (Berl.) 37: 433–444 (1972)

    Google Scholar 

  • Leenders, H.J., Knoppien, W.G.: Respiration of larval salivary glands of Drosophila in relation to the activity of specific genoma loci. J. Insect Physiol. 19, 1793–1800 (1973)

    Google Scholar 

  • Leenders, H.J., Derkson, J., Maas, P.M.J.M., Berendes, H.D.: Selective induction of a giant puff in Drosophila hydei by vitamin B6 and derivatives. Chromosoma (Berl.) 41, 447–460 (1973)

    Google Scholar 

  • Le Sturgeon, W.M., Beyer, A.L.: The rapid isolation, high resolution electrophoretic characterization and purification of nuclear proteins. In: Methods in cell biology vol 14, pp. 387–406. New York: Academic Press Inc. 1977

    Google Scholar 

  • Lewis, M., Helmsing, P.J., Ashburner, M.: Parallel changes in puffing activity and patterns of protein snythesis in salivary glands of Drosophila. Proc. nat. Acad. Sci. (Wash.) 72, 3604–3608 (1975)

    Google Scholar 

  • McKenzie, S.L., Henikoff, S., Meselson, M.: Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster. Proc. nat. Acad. Sci. (Wash.) 72, 1117–1121 (1975)

    Google Scholar 

  • Poels, C.L.M.: Mucopolysaccaride secretion from Drosophila salivary gland cells as a consequence of hormone induced gene activity. Cell Different. 1, 63–78 (1972)

    Google Scholar 

  • Ritossa, F.: A new puffing pattern induced by heat shock and DNP in Drosophila. Experientia (Basel) 18, 571–573 (1962)

    Google Scholar 

  • Ritossa, F.M.: Experimental activation of specific loci in polytene chromosomes of Drosophila. Exp. Cell Res. 35, 601–607 (1964)

    Google Scholar 

  • Sondermeijer, P.J.A., Lubsen, N.H.: The activity of two heat shock loci of Drosophila hydei in tissue culture cells and salivary gland cells as analyzed by in situ hybridization of complimentary DNA. Chromosoma (Berl.) 72, 281–291 (1979)

    Google Scholar 

  • Spradling, A., Penman, S., Pardue, M.L.: Analysis of Drosophila mRNA by in situ hybridization: sequences transcribed in normal and heat shocked cultured cells. Cell 4, 395–404 (1975)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brady, T., Belew, K. Pyridoxine induced puffing (II-48 C) and synthesis of a 40 KD protein in Drosophila hydei salivary glands. Chromosoma 82, 89–98 (1981). https://doi.org/10.1007/BF00285751

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00285751

Keywords

Navigation