Summary
Satellite DNA III becomes visualized by staining chromosomes with Giemsa at pH 10–12. Evidence is presented that besides the secondary constriction of chromosome 9, satellite III is contained in considerable amount in the long arms of chromosome 20, giving rise to a clearly visible secondary constriction just below the centromere. The latter finding confirms that reported by Bobrow et al. (1972).
The long arms of the Y chromosome also show strong staining with alkaline Giemsa, the region of staining corresponding exactly with the intensely fluorescing area. This is interpreted as possible evidence for the presence of satellite DNA III in the distal long arms of the human Y chromosome.
Zusammenfassung
Satelliten-DNA III scheint durch Färbung mit Giemsa bei einem pH von 10–12 sichtbar zu werden. Außer in der Sekundärkonstriktion von Chromosom 9 ist Satelliten-DNA III in hinreichender Menge im langen Arm von Chromosom 20 vorhanden, da nach Färbung mit Giemsa-12 eine deutliche Sekundärkonstriktion sichtbar wird. Dieser Befund wurde schon von Bobrow et al. erhoben (1972).
Die langen Y-Arme färben sich auch stark mit alkalischem Giemsa; die angefärbte Region stimmt genau mit dem intensiv fluorescierenden Anteil überein. Möglicherweise ist dies ein Hinweis für die Anwesenheit von Satelliten-DNA III im distalen Langarm des menschlichen Y-Chromosoms.
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References
Bobrow, M., Madan, K.: The effects of various banding procedures on human chromosomes studied with acridine organge. Cytogenet. Cell Genet. 12, 145–156 (1973)
Bobrow, M., Madan, K., Pearson, P. L.: Staining of some specific regions of human chromosomes, particularly the secondary constriction of No. 9. Nature (Lond.) New Biol. 238, 122–124 (1972)
Corneo, G., Zardi, L., Polli, E.: Elution of human satellite DNA's on a methylated albumin kieselguhr chromatographic column. Biochim. biophys. Acta (Amst.) 269 201–204 (1972)
Evans, H. J.: Molecular architecture of human chromosomes. Brit. med. Bull. 196–203 (1973)
Gagné, R., Laberge, C.: Specific cytological recognition of the heterochromatic segment of number 9 chromosome in man. Exp. Cell Res. 73, 239–242 (1972)
Gall, J. G., Pardue, M. L.: Methods in nucleic acid hybridization in cytological preparations. In: Methods in enzymology (ed. S. P. Colowick, N. O. Kaplan), Vol. 21, pp. 470–480. New York: Academic Press 1971
Jones, K. W.: Satellite DNA. J. med. Genet. 10, 273–281 (1973)
Jones, K. W., Corneo, G.: Location of satellite and homogenous DNA sequences on human chromosomes. Nature (Lond.) New Biol. 233, 268–271 (1971)
Jones, K. W., Prosser, J., Corneo, G., Ginelli, E.: The chromosomal location of human satellite DNA III. Chromosoma (Berl.) 42, 445–451 (1973)
Saunders, G. F., Hsu, T. C., Getz, M. J., Simes, E. L., Arrighi, F. E.: Locations of a human satellite DNA in human chromosomes. Nature (Lond.) New Biol. 236, 244–246 (1972)
Selander, R. K., De la Chapelle, A.: The fluorescence of quinacrine mustard with nucleic acids. Nature (Lond.) New Biol. 245, 240–243 (1973)
Evans, H. J., Gosden, J. R., Mitchell, A. R., Buckland, R. A.: Location of human satellite DNAs on the Y chromosome. Nature (Lond.) 251, 346–347 (1974)
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Bühler, E.M., Tsuchimoto, T., Jurik, L.P. et al. Satellite DNA III and alkaline Giemsa staining. Hum Genet 26, 329–333 (1975). https://doi.org/10.1007/BF00285384
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DOI: https://doi.org/10.1007/BF00285384