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In vivo synthesis of acid mucopolysaccharides by Ehrlich ascites tumor cells

Die Biosynthese von sauren Mucopolysacchariden in Ehrlich-Aszites-Tumorzellen

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Summary

The biosynthesis and intracellular distribution of acid mucopoly-saccharides in Ehrlich ascites tumor cells was studied in vivo by means of the precursors 35S-sulfate, 3H-glucosamine and 14C-galactosamine. It was found that the acid mucopolysaccharide present in the ascitic fluid supernatant is hyaluronic acid. Hyaluronic acid appears to be of extracellular origin, and it is bound to proteins of the cell membrane. The ascites cells exhibit a very active production of sulfated mucopolysaccharides particularly at the mitochondria and cell membrane level. Chondroitin sulfate A is the major component but also the isomers B and C are present. The possible role of chondroitin sulfate A in the development of neoplastic characteristics of the cell is discussed.

Zusammenfassung

Die Biosynthese und intrazelluläre Verteilung von sauren Mucopolysacchariden in Ehrlich-Aszites-Tumorzellen wurde in vivo mit Hilfe der Vorstufen 35S-Sulfat, 3H-Glucosamin und 14C-Galactosamin untersucht. Hyaluronsäure ist das Mucopolysaccharid, das in der überstehenden Aszites-Flüssigkeit anwesend ist und offenbar nicht in den Asziteszellen entsteht. Hyaluronsäure von extrazellulärem Ursprung ist wahrscheinlich an Proteine der Zellmembrane gebunden. Die Asziteszellen zeigen eine sehr rege Bildung von sulfatierten Mucopolysacchariden, besonders an den Mitochondrien und den Zellmembranen. Die wesentliche Komponente ist Chondroitin Sulfat A, jedoch findet man auch die Isomere B und C. Die mögliche Rolle von Chondroitin Sulfat A bei der Entwicklung der neoplastischen Eigenschaften der Zelle wird diskutiert.

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Authors and Affiliations

  1. Innere Klinik und Poliklinik, Tumorforschung, Universitätsklinikum der GHS Essen, Hufelandstraße 55, D-4300, Essen 1, Federal Republic of Germany

    L. J. Anghileri

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  1. L. J. Anghileri
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This work was supported by a grant from the Ministerium für Wissenschaft und Forschung des Landes Nordrhein-Westfalen

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Anghileri, L.J. In vivo synthesis of acid mucopolysaccharides by Ehrlich ascites tumor cells. Z. Krebsforsch. 88, 17–24 (1976). https://doi.org/10.1007/BF00284743

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