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Localisation ultrastructurale de l'activite de la phosphatase alcaline dans le foie foetal humain

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Summary

A direct lead method for visualization of alkaline phosphatase activity at pH 8 was used in an electron microscope study of the foetal development of human liver.

An accumulation of lead phosphate, the final product of the enzymatic reaction, was observed on the cell membranes of hepatocytes and also on those of erythroblasts, especially in immature cells.

An intense reaction was observed in peribiliary granules and in granules of the erythropoietic cells.

The localization of lead phosphate precipitate along the cell membranes and in some cytoplasmic bodies is discussed in relation to the procedure used.

Résumé

Les auteurs, dans le cadre d'une étude ultrastructurale du foie foetal humain, ont utilisé une méthode directe à pH 8 pour mettre en évidence l'activité de la phosphatase alcaline.

L'accumulation du produit final de la réaction histochimique (phosphate de plomb) a pu être observée de façon constante sur les membranes plasmatiques des hepatocytes et des érythroblastes. Dans la lignée érythropoïétique les dépôts sont toujours plus intenses sur les cellules les plus immatures.

Les auteurs ont observé également des dépôts importants de phosphate de plomb dans certains ≪granules≫ péribiliaires ainsi que dans des ≪granules≫ des cellules érythropoïétiques.

La disposition des dépôts le long des membranes cellulaires et dans certains organites cytoplasmiques est discutée en fonction de la technique de mise en évidence.

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Authors and Affiliations

  1. Centre de Microscopie Electronique Institut d'Anatomie Pathologique Université de Lausanne, Suisse

    R. Picardi, D. Gardiol & A. Gautier

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  1. R. Picardi
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  2. D. Gardiol
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  3. A. Gautier
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Ce travail a été effectué avec l'aide du ≪ Fonds National Suisse de la Recherche Scientifique ≫.

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Picardi, R., Gardiol, D. & Gautier, A. Localisation ultrastructurale de l'activite de la phosphatase alcaline dans le foie foetal humain. Histochemie 9, 58–67 (1967). https://doi.org/10.1007/BF00281807

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  • DOI: https://doi.org/10.1007/BF00281807

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