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Studies on isozymes of sorbitol dehydrogenase in some vertebrate species

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Summary

In swine, the enzyme sorbitol dehydrogenase exhibits a genetically determined polymorphism as identified by multiple electrophoretic bands suggesting a tetrameric structure (Op't Hof, 1969). In order to obtain further information on the genetics of this polymorphism, a number of other vertebrate species were examined. Multiple alleles at the SDH gene locus were found in the Cyprinid fishes Leuciscus and Rutilus, and in the Salmonid fish Salmo trutta. The electrophoretic patterns found in these species are compatible with the model of a tetrameric structure of the enzyme. This model was further verified by dissociation-reassociation studies with the homomeric enzymes of swine and Leuciscus. Since in the heterozygote an intensity gradient of the isozymes was seen, the three phenotypes observed in swine were further characterized by means of activity measurements and application of different substrates. The results point to a differential activity of the two different homomeres.

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Authors and Affiliations

  1. Institut für Humangenetik und Anthropologie der Universität Freiburg i. Br., Freiburg i. Br., Deutschland

    J. Op't Hof, U. Wolf & W. Krone

Authors
  1. J. Op't Hof
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  2. U. Wolf
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  3. W. Krone
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Supported by the Deutsche Forschungsgemeinschaft.

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Op't Hof, J., Wolf, U. & Krone, W. Studies on isozymes of sorbitol dehydrogenase in some vertebrate species. Hum Genet 8, 178–182 (1969). https://doi.org/10.1007/BF00280570

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  • DOI: https://doi.org/10.1007/BF00280570

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