Abstract
We have found a cruciform cutting endonuclease in the yeast, Saccharomyces cerevisiae, which localizes to the mitochondria. This activity apparently is associated with the mitochondrial inner membrane since the activity is not released into solution by osmolysis, in contrast to the matrix enzyme, isocitrate dehydrogenase. The cruciform cutting activity appears to be encoded by CCE1. This gene has been shown to encode one of the major cruciform cutting endonucleases present in a yeast cell. In ccel strains, which lack CCE1 endonuclease activity, the mitochondrial cruciform cutting endonucleolytic activity is also absent. Since CCE1 is allelic to MGT1, a gene required for the highly biased transmission of petite mitochondrial DNA in crosses between ϱ+ and hypersuppressive ϱ− cells, it seems likely that the CCE1 endonuclease functions within mitochondria.
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Blanc H, Dujon B (1986) Replicator regions of the yeast mitochondrial DNA responsible for suppressiveness. Proc Natl Acad Sci USA 77:3942–3946
Dake E, Hofmann TJ, McIntire S, Hudson A, Zassenhaus HP (1988) Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae. J Biol Chem 263:7691–7702
de Massy B, Weisberg RA, Studier FW (1987) Gene 3 endonuclease of bacteriophage T7 resolves conformationally branched structures in double-stranded DNA. J Mol Biol 193:359–376
Dressler D, Potter H (1982) Molecular mechanisms in genetic recombination. Annu Rev Biochem 51:727–761
Dunderdale HJ, Benson FE, Parsons CA, Sharples GJ, Lloyd RG, West SC (1991) Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins. Nature 354:506–510
Evans DH, Kolodner R (1987) Construction of a synthetic Holliday junction analog and characterization of its interaction with a Saccharomyces cerevisiae endonuclease that cleaves Holliday junctions. J Biol Chem 262:9160–9165
Evans DH, Kolodner R (1988) Effect of DNA structure and nucleotide sequence on Holliday junction resolution by a Saccharomyces cerevisiae endonuclease. J Mol Biol 201:69–80
Goto T, Laipis P, Wang JC (1984) The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae. J Biol Chem 259:10422–10429
Hsu PL, Landy A (1984) Resolution of synthetic att-site Holliday structures by the integrase protein of bacteriophage λ. Nature 311:721–726
Jensch F, Kosak H, Seeman NC, Kemper B (1989) Cruciform cutting endonucleases from Saccharomyces cerevisiae and phage T4 show conserved reactions with branched DNAs. EMBO J 8:4325–4334
Jeyaseelan R, Shanmugam G (1988) Human placental endonuclease cleaves Holliday junctions. Biochem Biophys Res Commun 155:1054–1060
Kleff S, Kemper B, Sternglanz R (1992) Identification and characterization of yeast mutants and the gene for a cruciform cutting endonuclease. EMBO J 11:699–704
Kornberg A (1955) Isocitric dehydrogenase of yeast. Methods Enzymol 1:705–709
Krebs EG (1955) Glyceraldehyde-3-phosphate dehydrogenase from yeast. Methods Enzymol 1:407–411
Mason TL, Poynton RO, Wharton DC, Schatz G (1973) Cytochrome c oxidase from baker's yeast. J Biol Chem 248:1346–1349
Orr-Weaver TL, Szostak JW (1985) Fungal recombination. Microbiol Rev 49:33–58
Smith GR (1988) Homologous recombination in procaryotes. Microbiol Rev 52:1–28
Symington LS, Kolodner R (1985) Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions. Proc Natl Acad Sci USA 82:7247–7251
Van der Wilden W, Matile P, Schellenberg M, Meyers J, Wiemken A (1973) Vacuolar membranes: isolation from yeast cells. Z Naturforsch 28C:416–419
West SC, Korner A (1985) Cleavage of cruciform DNA structures by an activity from Saccharomyces cerevisiae. Proc Natl Acad Sci USA 82:6445–6449
Zassenhaus HP, Hofmann TJ, Uthayashanker R, Vincent RD, Zona M (1988) Construction of a yeast mutant lacking the mitochondrial nuclease. Nucleic Acids Res 16:3283–3296
Zweifel SG, Fangman WL (1991) A nuclear mutation reversing a biased transmission of yeast mitochondrial DNA. Genetics128:241–249
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Communicated by D. Lonsdale
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Ezekiel, U.R., Zassenhaus, H.P. Localization of a cruciform cutting endonuclease to yeast mitochondria. Molec. Gen. Genet. 240, 414–418 (1993). https://doi.org/10.1007/BF00280395
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DOI: https://doi.org/10.1007/BF00280395