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Organization and functional analysis of three T-DNAs from the vitopine Ti plasmid pTiS4

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Summary

The vitopine Ti plasmid pTiS4 of Agrobacterium vitis has an unusual T-DNA organization. The pTiS4 oncogenes, localized by screening selected pTiS4 clones for growth-inducing activity, are localized on three T-DNAs, whereas in all other characterized Ti plasmids one or two T-DNAs are found. The nucleotide sequences and predicted amino acid sequences of the pTiS4 oncogenes set them apart from the corresponding genes from other Ti or Ri plasmids. The oncogenes induce the same type of reaction on various test plants as the well-known pTiAch5 oncogenes but the pTiS4 ipt gene induces considerably more shoots than its Ach5 homologue. We have also identified the gene coding for vitopine synthase as well as a vitopine synthase pseudogene. Both sequences show homology to the octopine synthase gene. In terms of both nucleotide sequence and overall organization, the pTiS4 T-DNAs appear to be only distantly related to previously characterized T-DNAs.

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Abbreviations

Ap:

ampicillin

IS:

insertion sequence

iaaH :

indole acetamide hydrolase

iaaM :

tryptophan monooxygenase

ipt :

isopentenyl transferase

Km:

kanamycin

LB:

Luria broth

m/a:

mannopine/agropine

o/c:

octopine/cucumopine

ocs :

octopine synthase

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Authors and Affiliations

  1. Institut de Biologic Moléculaire des Plantes du CNRS, 12 rue du Général Zimmer, 67084, Strasbourg, France

    Jean Canaday, Jean-Claude Gérard, Philippe Crouzet & Léon Otten

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  1. Jean Canaday
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  2. Jean-Claude Gérard
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  3. Philippe Crouzet
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  4. Léon Otten
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Communicated by A. Kondorosi

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Canaday, J., Gérard, JC., Crouzet, P. et al. Organization and functional analysis of three T-DNAs from the vitopine Ti plasmid pTiS4. Molec. Gen. Genet. 235, 292–303 (1992). https://doi.org/10.1007/BF00279373

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