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Elektronenmikroskopische Untersuchungen der lysosomalen sauren Phosphatase in den Keimblättern des Ratten-Embryo von Tag 8–10

Electron microscopic studies on the lysosomal acid phosphatase in the germ layers of the rat embryo of day 8–10

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Summary

Acid phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.2) has been studied electron microscopically in the rat embro between day 8 and 10 of gestation. In the visceral yolk sac epithelium phagosomes, acid phosphatase containing inclusions (lysosomes) and phago-lysosomes are increased shortly before the development of the embryo shape in the middle of day 9. The parietal entodermal cells contain ribosome-coated endoplasmatic reticulum, secretory granules and crino-lysosomes. After the development of the mesoderm lysosomes also occur in the embryonic ectoderm. At day 9 and 14 hours fetal erythroblasts and vessels are formed in the mesoderm. Cyto-lysosomes have not been found.

Zusammenfassung

Die saure Phosphatase (Orthophosphosäure Monoester Phosphohydrolase, EC 3.1.3.2) wurde elektronenmikroskopisch im Ratten-Embryo zwischen Tag 8 und 10 der Schwangerschaft untersucht. Im visceralen Dottersackepithel treten vermehrt Phagosomen, saure Phosphatase enthaltende Einschlüsse (Lysosomen) und Phago-Lysosomen kurz vor der Ausbildung der Embryonalgestalt in der Mitte des Tages 9 auf. Die parietalen Entodermzellen enthalten Ribosomen-besetztes endoplasmatisches Retikulum, Sekretgranula und Krino-Lysosomen. Nach der Entwicklung des Mesoderm kommen auch im embryonalen Ektoderm vereinzelt Lysosomen vor. Am Tag 9 und 14 Std entwickeln sich im Mesoderm fetale Erythroblasten und Gefäße. Cyto-Lysosomen treten nicht auf.

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Authors and Affiliations

  1. Forschungsabteilung für Elektronenmikroskopie, Deutschland

    S. Spors

  2. Pharmakologisches Institut der Freien Universität Berlin, Deutschland

    S. Spors

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  1. S. Spors
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Spors, S. Elektronenmikroskopische Untersuchungen der lysosomalen sauren Phosphatase in den Keimblättern des Ratten-Embryo von Tag 8–10. Histochemie 25, 143–151 (1971). https://doi.org/10.1007/BF00279112

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  • DOI: https://doi.org/10.1007/BF00279112

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