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Rapid determination of hypoxanthine-guanine-phosphoribosyl transferase in human fibroblasts and amniotic cells

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Summary

A micromodification of the method of HGPRT and APRT assay is described, which measures the incorporation of 14C hypoxanthine and 14C adenine into cultured skin fibroblasts and amniotic cells grown on microtiter plates. Only about 10 000 cells are needed per assay. By this method HGPRT deficient cells can be easily distinguished from normal cells. Investigations with respect to the effect of substrate concentrations and time of incubation have been carried out on some normal fibroblast cell lines, amniotic cell lines and 3 Lesch-Nyhan cell lines.

Another modified method is described for quantitative determination of HGPRT activity by means of radio thin-layer chromatography.

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Authors and Affiliations

  1. Institute of Human Genetics, University of Hamburg, Germany

    I. Willers, D. P. Agarwal, S. Singh, W. Schloot & H. W. Goedde

Authors
  1. I. Willers
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  2. D. P. Agarwal
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  3. S. Singh
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  4. W. Schloot
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  5. H. W. Goedde
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Supported by the Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg.

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Willers, I., Agarwal, D.P., Singh, S. et al. Rapid determination of hypoxanthine-guanine-phosphoribosyl transferase in human fibroblasts and amniotic cells. Hum Genet 27, 323–328 (1975). https://doi.org/10.1007/BF00278425

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  • DOI: https://doi.org/10.1007/BF00278425

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