Summary
The distribution of adrenaline, noradrenaline, aliesterases and non-specific cholinesterases in the cortical and medullary cells and that of ascorbic acid in the cortex have been studied histochemically in sections of adrenal glands from embryonic, juvenile and adult chicken. Both the catecholamines are secreted by the embryonic medulla from the 11th day of incubation but noradrenaline is the more abundant of the two hormones at all stages and it is secreted by the majority of chromaffin cells. There is a tendency for the adrenaline-secreting cells to predominate in the subcapsular layer of the medulla. Both types of chromaffin cells reveal considerable cholinesterase activity consistently from the second half of incubation period onwards.
A high concentration of aliesterases and ascorbic acid are developed and maintained in the cortical cords from the time the cortex begins secretory activity, namely, the 10-day incubation stage. Lower concentrations of cholinesterases are also present in the cells of the cortex. The cords of the peripheral zone of cortex show higher concentrations of both the enzymes and ascorbic acid than those of the central zone.
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Allen, J. M.: A chemical and histochemical study of aliesterase in mouse adrenal cortex under hormonal influence and altered sodium intake. Endocrinology 61, 368–374 (1957).
—: A chemical and histochemical study of aliesterase in the adrenal gland of the developing mouse. Anat. Rec. 132, 195–208 (1958).
Arvy, L.: Contribution à l'histochimie de la glande surrénale chez Gallus domesticus L. et chez Anas boschas. C.R. Soc. Biol. (Paris) 155, 69–71 (1961).
Bacchus, H.: A cytochemical study of the adrenal cortex of the rat under salt stresses. Amer. J. Physiol. 163, 326–331 (1950).
Bourne, G.: Recent discoveries concerning mitochondria and Golgi apparatus and their significance in cellulose physiology. J. roy. micr. Soc. 70, 367–379 (1950).
Brauer, A.: A topographical and cytological study of the sympathetic nervous components of the suprarenal of the chick embryo. J. Morph. 53, 277–325 (1932).
Chavin, W., and A. Kovacevic: Adrenocortical histochemistry of intact and hypophysectomized goldfish. Gen. comp. Endocr. 1, 264–274 (1961).
Dawson, A. B.: Histochemical evidence of early differentiation of the suprarenal gland of the chick. J. Morph. 92, 579–596 (1953).
Deane, H. W., B. L. Rubin, E. C. Driks, B. L. Lobel, and G. Leipsner: Trophoblastic giant cells in placentas of rats and mice and their probable role in steroid hormone production. Endocrinology 70, 407–419 (1962).
Eränkö, O.: Distribution of adrenaline and noradrenaline in the adrenal medulla. Nature (Lond.) 175, 88–89 (1955).
—: Distribution of adrenaline and noradrenaline in the hen adrenal medulla. Nature (Lond.) 179, 417–418 (1957).
Falck, B., N.-Å, Hillarp, and B. Högberg: Content and intracellular distribution of adenosine triphosphate in cow adrenal medulla. Acta physiol. scand. 36, 360–376 (1956).
Ghosh, A.: A comparative study of the histochemistry of the avian adrenals. Gen. comp. Endocr., Suppl. 1, 75–80 (1962a).
—, and A. Ghosh: A comparative cytochemical study of the avian suprarenal medulla. Proc. zool. Soc. 15, 135–145 (1962).
Gomori, G.: In: Menstruation and its disorders. Edit. E. T. Engle (1950).
—: Microscopic histochemistry, principles and practice. Chicago: Chicago Univ. Press 1952.
Hillarp, N.-Å.: Enzymic systems involving adenosine triphosphates in the adrenaline and noradrenaline containing granules of the adrenal medulla. Acta physiol. scand. 42, 144–165 (1958).
—, and B. Hökfelt: Histochemical demonstration of noradrenaline and adrenaline in the adrenal medulla. J. Histochem. Cytochem. 3, 1–5 (1955).
— and G. Thieme: Adenosine triphosphates in the adrenal medulla. Acta physiol. scand. 47, 328–338 (1959).
Kamo, M.: Effect of various choline esters upon the secretion rate of adrenaline and noradrenaline of the adrenal gland. Tohoku J. exp. Med. 76, 319–325 (1962).
Kar, A.: Studies on distribution and concentrations of alkaline phosphatase in adrenals of normal and sex-hormone treated pigeons. Endocrinology 46, 363–366 (1950).
Kersten, W., S. Leonhäuser, and H. Staudinger: Zum Wirkungsmechanismus der Ascorbinsäure II. Ascorbinsäure-abhängige DPNH-oxydase und Hydroxylierungsreaktionen bei Steroiden. Biochem. biophys. Acta (Amst.) 29, 350–358 (1958).
—, H. Schmidt, and H. Staudinger: Stoffwechsel der Nebennierenrinde und Biosynthese der Corticosteroide. Ascorbinsäure und Wasserstofftransport. Biochem. Z. 326, 469–473 (1955).
Mahon, E. F., W. S. Hoar, and S. Tabata: Histophysiological studies of the adrenal tissues of the goldfish. Canad. J. Zool. 40, 449–469 (1962).
Oguri, M.: Studies on the adrenal gland of teleostsIV. Histochemistry of the interrenal cells of fishes. Bull. Japan. Soc. Sci. Fisheries 26, 447–451 (1960).
Okuda, M.: The adrenaline content of the suprarenal capsule in the chick embryo. Endocrinology 8, 342–348 (1928).
Pearse, A. G. E.: Histochemistry, theoretical and applied. London: J. & A. Churchill 1960.
Prusoff, W. H., H. Blaschko, M. G. Ord, and L. A. Stocken: Incorporation of phosphorus 32 into adenosine triphosphate of adrenal chromaffin granules. Nature (Lond.) 190, 354–355 (1961).
Shepherd, D. M., and G. B. West: Noradrenaline and the adrenal medulla. Brit. J. Pharmacol. 6, 665–674 (1951).
Sivaram, S.: Histochemistry of the developing adrenal gland of the chick. Anat. Rec. 148, 336 (1964a).
—: Histochemistry of some phosphatases in the developing adrenal gland of Gallus domesticus. Seminar on “Research trends in animal morphology” — Publication by Vikram University, Ujjain, India (in press, 1965).
—: Structure and development of the adrenal gland of Gallus domesticus. Canad. J. Zool. 43, 1021–1031 (1965a).
—, and D. R. Sharma: Beta metachromasia of the chromaffin tissue of chick adrenal. J. Histochem. Cytochem. 12, 852–853 (1964).
Sun, T. P.: Histophysiological study of the glands of internal secretion and their effect on ossification in the chondrodystrophic and “sticky” chicken embryo. Physiol. Zool. 5, 375–383 (1932).
Wood, J. G.: Identification of and observations on epinephrine and norepinephrine containing cells in the adrenal medulla. Amer. J. Anat. 112, 285–304 (1963).
Wright, A., and I. Chester Jones: Chromaffin tissue in the lizard adrenal medulla. Nature (Lond.) 175, 1001–1002 (1955).
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From a thesis submitted to McGill University, Montreal, Canada in 1963 in partial fulfillment of the requirements for the degree of Doctor of Philosophy. The work was done during tenure of a Canadian Commonwealth Scholarship.
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Sivaram, S. Histochemical studies on the developing adrenal gland of Gallus domesticus . Histochemie 12, 316–325 (1968). https://doi.org/10.1007/BF00278304
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DOI: https://doi.org/10.1007/BF00278304