Summary
Elastic fibres may be stained by a number of dyes, e.g. Direct Blue 1 (C.I. 24410), Direct Blue 10 (C.I. 24340), Direct Blue 15 (C.I. 24400), Direct Blue 152 (C.I. 24366) and Direct Violet 37 (C.I. 24370). A convenient method using Direct Blue 152 has been developed which is specific for elastic fibres. The method is simple and allows the demonstration of other connective tissue fibres. Staining of elastic fibres is unimpaired by numerous blocking procedures or by changes in dyebath pH. These properties are shared by several standard elastic fibre stains.
As the Direct dyes and several of the standard elastic fibre stains possess numerous aromatic rings a wide range of dyes containing varying numbers of aromatic rings were examined for ability to stain elastic fibres. No association was observed between the ability to stain elastic fibres and dye class, formal charge or the presence of hydrogen bonding groups. Staining was, however, definitely associated with the presence in the dye molecule of 5 or more aromatic rings. This suggested that van der Waals forces of attraction may be responsible for elastic fibre staining both by Direct dyes and the standard elastic fibre stains. Staining of elastic fibres as a side-effect in many procedures is similarly explicable.
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References
Arshid, F. M., Connelly, R. F., Desai, J. N., Fulton, R. G., Giles, C. H., Kefalis, J. C.: A study of certain natural dyes. Part 2. The structure of the metallic lakes of Brazilwood and Logwood colouring matters. J. Soc. Dyers Colour. 70, 402–412 (1951).
Ayer, J. P.: Elastic tissues. Int. Rev. Cont. Tissue Res. 2, 33–100 (1964).
Baker, J. R.: Principles of biological microtechnique. London: Methuen & Co. Ltd. 1958.
Ballantyne, B.: Pseudo-positive elastin reaction with the naphthoic acid hydrazide technique for monoamine oxidase. Ann. Histochim. 13, 195–204 (1968).
Bangle, R.: Gomori's paraldehyde-fuchsin stain. I. Physico chemical and staining properties of the dye. J. Histochem. Cytochem. 2, 291–299 (1954).
Berenbaum, M. C.: The histochemistry of bound lipids. Quart J. micr. Sci. 99, 231–242 (1958).
Dempsey, E. W., Vial, J. D., Lucas, R. V., Lansing, A. I.: Characterisation of the reaction between orcein and the elastic fibres of the ligamentum nuchae of the horse. Anat. Rec. 113, 197–214 (1952).
Friedberg, S. H., Goldstein, D. J.: Thermodynamics of orcein staining of elastic fibres. Histochem. J. 1, 361–376 (1969).
Fullmer, H. M.: The histochemistry of the connective tissues. Int. Rev. Conn. Tissue Res. 3, 1–76 (1965).
—, Lillie, R. D.: Some aspects of the mechanism of orcein staining. J. Histochem. Cytochem. 4, 64–68 (1956).
—: The staining of collagen with elastic tissue stains. J. Histochem. Cytochem. 5, 11–14 (1957).
—: The oxytalan fibre: a previously undescribed connective tissue fibre. J. Histochem. Cytochem. 6, 425–430 (1958).
Geyer, G.: About histochemistry of elastin. Studies on the detection of desmosins. Acta histochem. (Jena) 34, 257–262 (1969).
Giles, C. H., Hassan, A. S. A.: Adsorption at organic surfaces. Part 5. A study of the adsorption of dyes and other organic solutes by cellulose and chitin. J. Soc. Dyers Colour. 74, 846–857 (1958).
Goldstein, D. J.: Ionic and non-ionic bonds in staining, with special reference to the action of urea and sodium chloride on the staining of elastic fibres and glycogen. J. roy. micr. Soc. 103, 477–492 (1962).
—: Correlation of size of dye particle and density of substrate, with special reference to mucin staining. Stain Technol. 37, 79–93 (1962).
—: Selective staining of eosinophil granules in sections by alkaline orcein in a concentrated urea solution. Stain Technol. 38, 49–51 (1963).
Gomori, G.: Aldehyde-fuchsin: a new stain for elastic tissue. Amer. J. clin. Path., 20, 665–666 (1950).
Hall, D. A.: The chemistry of connective tissue. Springfield: Ch. C. Thomas 1961.
Haurowitz, F.: The chemistry and function of proteins, 2nd ed. London: Academic Press 1963.
Hickinbottom, W. J.: Reactions of organic compounds, 3rd ed. London: Longmans, Green & Co. 1957.
Hinshelwood, C. N.: The structure of physical chemistry. Oxford: Oxford University Press 1951.
Leach, E. H.: Bismark Brown as a stain for mucoproteins. Stain Technol. 23, 73–76 (1947).
Lillie, R. D.: Histopathologic technic and practical histochemistry. New York: McGraw-Hill Book Co. 1965.
—: Exploration of dye chemistry in Taenzer Unna Orcein type elastin staining. Histochemie 19, 1–12 (1969).
Mander, S. T. K., Mander, L. N., Carmichael, G. G.: The staining mechanism of aldehydefuchsin, with respect to the oxytalan fibre in the mouse. J. Histochem. Cytochem. 16, 480–485 (1968).
Michaelis, L.: On the chemistry of elastic stains and its practical application in the case of sputum preparations. Dtsch. med. Wschr. 22, 219 (1901). Cit. in: Z. wiss. Mikr. 18, 310–311.
Monis, B.: The staining of elastic fibres with certain metal cyanide complexes and basic dyes, with some histochemical observations. J. Histochem. Cytochem. 3, 408–409 (1955).
Mukerjee, P., Ghosh, A. K.: The effect of urea on Methylene Blue, its self association and interaction with polyelectrolytes in aqueous solution. J. Phys. Chem. 67, 193–197 (1963).
Musso, H.: Orcein and litmus dyes. Composition and constitution confirmed by synthesis. Plant Med. 8, 432–446 (1960).
Nakao, K., Angrist, A. A.: A histochemical demonstration of aldehyde in elastin. Amer. J. clin. Path. 49, 65–67 (1968).
Newton, A. V.: Collagen, elastic and resorcin-fuchsin. Nature (Lond.) 209, 1146–1147 (1966).
Paget, G. E.: Aldehyde-Thionin: a stain having similar properties to aldehyde-fuchsin. Stain Technol. 34, 223–226 (1959).
Puchtler, H., Sweat, F., Bates, R., Brown, J. H.: On the mechanism of resorcin-fuchsin staining. J. Histochem. Cytochem. 9, 553–559 (1961).
Robinson, D. W., Jencks, W. P.: The effect of compounds of the urea-guanidinium class on the activity coefficient of acetylated glycine ethyl ester and related compounds. J. Amer. chem. Soc. 87, 2462–3470 (1965).
Rodd, E. H.: Chemistry of carbon compounds. Amsterdam: Elsevier 1965.
Salthouse, T. N.: Selective staining of collagen and elastin by Luxol Fast Blue G in methanol. J. Histochem. Cytochem. 13, 133–140 (1965).
Singer, M.: Staining of tissue sections with acid and basic dyes. Internat. Rev. Cytol. 1, 211–255 (1952).
Spicer, S. S., Lillie, R. D.: Histochemical identification of basic protein with Biebrich scarlet at alkaline pH. Stain Technol. 36, 365–370 (1961).
Sumner, B. E. H.: A histochemical study of aldehyde-fuchsin staining. J. roy. micr. Soc. 84, 329–338 (1965).
Terner, J. Y., Gurland, J., Gaer, F.: Phosphotungstic acid-haematoxylin: spectrophotometry of the lake in solution and stained tissue. Stain Technol. 39, 141–153 (1964).
Thompson, S. W.: Selected histochemical and histopathological methods. Springfield: Ch. C. Thomas 1966.
Verhoeff, F. H.: Some new staining methods of wide applicability; including a rapid differential stain for elastic tissue. J. Amer. med. Ass. 50, 876–877 (1908).
Weigert, C.: A method for staining elastic fibres. Zbl. allg. Path. path. Anat. 9, 289–292 (1898).
Weis, J.: The nature of the reaction between orcein and elastin. J. Histochem. Cytochem. 2, 21–28 (1954).
Wilcock, C. X., Ashworth, J. L.: Whittaker's dyeing with coal tar dyestuffs, 6th ed. London: Van Nostrand 1964.
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Horobin, R.W., James, N.T. The staining of elastic fibres with Direct Blue 152. A general hypothesis for the staining of elastic fibres. Histochemie 22, 324–336 (1970). https://doi.org/10.1007/BF00277460
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DOI: https://doi.org/10.1007/BF00277460