Summary
The yeast gene SNQ2 confers hyper-resistance to the mutagens 4-nitroquinoline-N-oxide (4-NQO) and Triaziquone, as well as to the chemicals sulphomethuron methyl and phenanthroline when present in multiple copies in transformants of Saccharomyces cerevisiae. Subcloning and sequencing of a 5.5 kb yeast DNA fragment revealed that SNQ2 has an open reading frame of 4.5 kb. The putative encoded polypeptide of 1501 amino acids has a predicted molecular weight of 169 kDa and has several hydrophobic regions. Northern analysis showed a transcript of 5.5 kb. Haploid cells with a disrupted SNQ2 reading frame are viable. The SNQ2-encoded protein has domains believed to be involved in ATP binding and is likely to be membrane associated. It most probably serves as an ATP-dependent permease.
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Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1988) Current protocols in molecular biology, vols. 1 & 2, chapters 2 and 13. Wiley Interscience, New York
Birnboim HG, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Chen C, Chin JE, Ueda K, Clark DP, Pastan I, Gottesman MM, Roninson IB (1986) Internal duplication and homology with bacterial transport proteins in the mdr1 (P-Glycoprotein) gene from multidrug-resistant human cells. Cell 47:381–389
Dagert M, Ehrlich SD (1979) Prolonged incubation in calcium chloride improves the competence of E. coli cells. Gene 6:23–28
Del Sal G, Manfioletti G, Schneider C (1988) A one-tube plasmid DNA mini-preparation suitable for sequencing. Nucleic Acids Res 16:9878
Doolittle RF, Johnson MS, Hussain J, Van Houten B, Thomas DC, Sancar A (1986) Domiainal evolution of a prokaryotic DNA repair protein and its relationship to active-transport proteins. Nature 323:451–453
Dreesen TD, Johnson DH, Henikoff S (1988) The brown protein of Drosophila melanogaster is similar to the white protein and to components of active transport complexes. Mol Cell Biol 8:5206–5215
Fleer R, Brendel M (1979) Formation and fate of crosslinks induced by polyfunctional anticancer drugs in yeast. Mol Gen Genet 176:41–52
Gömpel-Klein P, Brendel M (1990) Allelism of SNQ1 and ATR1, genes of the yeast Saccharomyces cerevisiae required for controlling sensitivity to 4-nitroquinoline-N-oxide and aminotriazole. Curr Genet 18:93–96
Gömpel-Klein P, Mack M, Brendel M (1989) Molecular characterization of two genes SNQ and SFA that confer hyperresistance of 4-nitroquinoline-N-oxide and formaldehyde in Saccharomyces cerevisiae. Curr Genet 16:65–74
Grey M, Brendel M (1992) Rapid and simple isolation of DNA from agarose gels. Curr Genet 22:83–84
Haase E, Servos J, Brendel M (1992) Isolation and characterization of additional genes influencing resistance to various mutagens in the yeast Saccharomyces cerevisiae. Curr Genet 21:319–324
Henikoff S (1987) Unidirectional digestion with exonuclease III in DNA sequence analysis. Methods Enzymol 15:156–165
Hertle K, Haase E, Brendel M (1991) The SNQ3 gene of Saccharomyces cerevisiae confers hyper-resistance to several functionally unrelated chemicals. Curr Genet 19:429–433
Higgins CF, Hyde SC, Mimmack MM, Gileadi U, Gill DR, Gallagher MP (1990) Binding protein-dependent transport systems. J Bioenerg Biomembr 22:571–592
Hill JE, Myers AM, Koerner TJ, Tzagoloff A (1986) Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast 2:163–167
Hussain M, Lenard J (1991) Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number. Gene 101:149–152
Hyde SC, Emsley P, Hartshorn MJ, Mimmack MM, Gileadi U, Pearce SR, Gallagher MP, Gill DR, Hubbard RE, Higgins CF (1990) Structural model of ATP-binding proteins associated with cystic fibrosis, multidrug resistance and bacterial transport. Nature 346:362–365
Ito H, Fukuda J, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163
Kanazawa S, Driscoll M, Struhl K (1988) ATR1, a Saccharomyces cerevisiae gene encoding a transmembrane protein required for aminotriazole resistance. Mol Cell Biol 8:664–673
Kane SE, Pastan I, Gottesman MM (1990) Genetic basis of multidrug resistance of tumor cells. J Bioenerg Biomembr 22:593–618
Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132
Leppert G, McDevitt R, Falco SC, Van Dyke TK, Ficke MB, Golin J (1990) Cloning by gene amplification of two loci conferring multiple drug resistance in Saccharomyces. Genetics 125:13–20
McGrath JP, Varshavsky A (1989) The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein. Nature 340:400–404
Moye-Rowley WS, Harshman KD, Parker CS (1989) Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins. Genes Dev 3:283–292
Rao MJK, Argos P (1986) A conformational preference parameter to predict helices in integral membrane proteins. Biochim Biophys Acta 869:197–214
Pepling M, Mount MS (1990) Sequence of a cDNA from the Drosophila melanogaster white gene. Nucleic Acids Res 18:1633
Rechsteiner M (1988) Regulation of enzyme levels by proteolysis: the role of PEST regions. Adv Enzyme Regul 27:135–151
Rechsteiner M, Rogers S, Rote K (1987) Protein structure and intracellular stability. Trends Biochem Sci 12:390–394
Riordan JR, Rommens JM, Kremm B-S, Alon N, Rozmahel R, Grzelczak Z, Zielenski J, Lok S, Plavsic N, Chou J-L, Drumm ML, Iannuzzi MC, Collins FS, Tsui L-C (1989) Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245:1066–1072
Rodriguez RL, Tait RC (1983) Recombinant DNA techniques: An introduction. Addison-Wesley, London
Rogers S, Wells R, Rechsteiner M (1986) Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis. Science 234:364–368
Rothstein RJ (1983) One-step gene disruption in yeast. Methods Enzymol 101:202–209
Ruhland AR, Brendel M (1979) Mutagenesis by cytostatic alkylating agents in yeast strains of differing repair capacities. Genetics 92:83–97
Ruhland AR, Haase E, Siede W, Brendel M (1981) Isolation of yeast mutants sensitive to the bifunctional alkylating agent nitrogen mustard. Mol Gen Genet 181:346–351
Sandbaken M, Lupisella JA, DiDomenico B, Chakraburtty K (1990) Isolation and characterization of the structural gene encoding elongation factor 3. Biochim Biophys Acta 1050:230–234
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Schnell N, Entian K-D (1991) Identification and characterization of a Saccharomyces cerevisiae gene (PAR1) conferring resistance to iron chelators. Eur J Biochem 200:487–493
Schnell N, Krems B, Entian K-D (1992) The PAR1 (YAP1) gene of Saccharomyces cerevisiae, a c-jun homologue, is involved in oxygen metabolism. Curr Genet 21:269–273
Sharp PM, Li WH (1987) The codon adaptation index — A measure of directional synonymous codon usage bias, and its potential applications. Nucleic Acids Res 15:1281–1295
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Communicated by C. Hollenberg
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Servos, J., Haase, E. & Brendel, M. Gene SNQ2 of Saccharomyces cerevislae, which confers resistance to 4-nitroquinoline-N-oxide and other chemicals, encodes a 169 kDa protein homologous to ATP-dependent permeases. Molec. Gen. Genet. 236, 214–218 (1993). https://doi.org/10.1007/BF00277115
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DOI: https://doi.org/10.1007/BF00277115