Summary
Biphasic insulin secretion from perifused rat islets of Langerhans was affected in three ways by the islet glucose environment prior to stimulation, (i) The secretory response to glucose was diminished if the basal concentration of glucose in the medium was reduced from 5.5 to 2.7 mmol/l for 2 h prior to stimulation. First phase secretion was affected more than the second, (ii) Secretion was potentiated if islets had been previously exposed to a stimulatory concentration of glucose of 22.2 mmol/l. Again first phase secretion was particularly affected and there was a positive correlation between the magnitude of the secretory response and the duration of the initial stimulus, (iii) In contrast, both phases of secretion were proportionately reduced if islets had been previously exposed to stimulatory concentrations of glucose of 8.3 mmol/l.
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References
Cerasi E, Luft R (1967) The plasma insulin response to glucose infusion in healthy subjects and in diabetes mellitus. Acta Endocrinol (Kbh) 55: 278–304
Porte D, Pupo A A (1969) Insulin responses to glucose: Evidence for a two pool system in man. J Clin Invest 48: 2309–2319
Grodsky G M, Bennett L L, Smith D, Nemechek K (1967) The effect of tolbutamide and glucose on the timed release of insulin from the isolated perfused pancreas. In: Butterfield W J H, Westering W (eds) Tolbutamide after ten years. Excerpta Medica Foundation, Amsterdam, p 11–21
Curry D L, Bennett L L, Grodsky G M (1968) Dynamics of insulin secretion by the perfused rat pancreas. Endocrinology 83: 572–584
Cerasi E, Fick G, Rudemo M (1974) A mathematical model for the glucose-induced insulin release in man. Eur J Clin Invest 4: 267–278
Rabinovitch A, Grill V, Renold A E, Cerasi E (1976) Insulin release and cyclic AMP accumulation in response to glucose in pancreatic islets of fed and starved rats. J Clin Invest 58: 1209–1216
Colwell J A, Lein A (1967) Diminished insulin response to hyperglycaemia in prediabetes and diabetes. Diabetes 16: 560–565
Serrano-Rios M, Ramos F, Rodriguez-Minon J L, Vivaneo F (1970) Studies in prediabetes. Insulin response to oral glucose, intravenous tolbutamide and rapid intravenous glucose infusion in genetic prediabetes. Diabetologia 6: 392–398
Cerasi E (1975) Potentiation of insulin release by glucose in man. I. Quantitative analysis of enhancement of glucose-induced insulin secretion by pretreatment with glucose in normal subjects. Acta Endocrinol (Kbh) 79: 483–501
Grodsky G M (1972) A threshold distribution hypothesis for packet storage of insulin and its mathematical modelling. J Clin Invest 51: 2047–2059
Cerasi E (1975) Feedback inhibition of insulin secretion in subjects with high and low insulin response to glucose. Diabete Metab 1: 73–76
Lacy P E, Kostianovsky M (1967) Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16: 35–39
Ashby J P, Speake R N (1975) Insulin and glucagon secretion from isolated islets of Langerhans: Effects of calcium ionophores. Biochem J 150: 89–96
Grill V, Adamson U, Cerasi E (1978) Immediate and time-dependent effects of glucose on insulin release from rat pancreatic tissue. Evidence for different mechanisms of action. J Clin Invest 61: 1034–1043
Hedeskov C J, Capito K (1974) Effect of starvation on insulin secretion and glucose metabolism in mouse pancreatic islets. Biochem J 140: 423–433
Ashcroft S J H, Weerasinghe C D, Randle P J (1973) Interrelationship of islet metabolism, adenosine triphosphate content and insulin release. Biochem J 132: 223–231
Frier B M, Corrall R J M, Ashby J P, Baird J D (in press) Attenuation of the pancreatic beta cell response to a meal following hypoglycaemia in man. Diabetologia
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Ashby, J.P., Shirling, D. Evidence for priming and inhibitory effects of glucose on insulin secretion from isolated islets of langerhans. Diabetologia 18, 417–421 (1980). https://doi.org/10.1007/BF00276824
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DOI: https://doi.org/10.1007/BF00276824