Abstracts
The periplasmic nitrate reductase was assayed in intact cells of Thiosphaera pantotropha, after aerobic growth with either malate, succinate, acetate, butyrate or caproate present as sole carbon source. The level of enzyme activity was largely dependent upon carbon source and was lowest on malate and succinate, intermediate on acetate and highest on butyrate and caproate. The presence or absence of nitrate did not effect enzyme activity. The results indicate that, during aerobic growth, activity of the periplasmic nitrate reductase increases with the extent of reduction of the carbon substrate.
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Abbreviations
- MV+ :
-
reduced methylviologen
References
Ballard AL, Ferguson SJ (1988) Respiratory nitrate reductase from Paracoccus denitrificans: evidence for two b-type haems in the γ-subunit and properties of a water-soluble active enzyme containing α- and β-subunits. Eur J Biochem 174: 207–212
Bell LC, Richardson DJ, Ferguson SJ (1990) Periplasmic and membrane-bound respiratory nitrate reductases in Thiosphaera pantotropha: the periplasmic enzyme catalyses the first step in aerobic denitrification. FEBS Lett 265: 85–87
Ferguson SJ (1987) Denitrification: a question of the control and organization of electron and ion transport. TIBS 12: 354–357
Robertson LA, Kuenen JG (1984) Aerobic denitrification: a controversy revived. Arch Microbiol 139: 351–354
Robertson LA, Kuenen JG (1989) Combined heterotrophic nitrification and aerobic denitrification in Thiosphaera pantotropha and other bacteria. Antonie van Leeuwenhoek 57: 139–152
Stewart V (1988) Nitrate respiration in relation to facultative metabolism in Enterobacteria. Microbiol Rev 52: 190–232
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Richardson, D.J., Ferguson, S.J. The influence of carbon substrate on the activity of the periplasmic nitrate reductase in aerobically grown Thiosphaera pantotropha . Arch. Microbiol. 157, 535–537 (1992). https://doi.org/10.1007/BF00276774
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DOI: https://doi.org/10.1007/BF00276774