Summary
A streptomycin-resistant cell line, SR155, was selected in a callus culture initiated from diploid Nicotiana sylvestris.
Regenerates of the SR155 line have a grossly altered morphology, an altered peroxidase isoenzyme pattern, and do not flower. The SR155 plants are diploid, but in the karyotype a chromosome translocation was detected.
Similar content being viewed by others
References
Binding, H.: Selektion in Kalluskulturen mit haploiden Zellen. Z. Pflanzenzüchtg. 67, 33–38 (1972)
Binding, H., Binding, K., Straub, J.: Selektion in Gewebekulturen mit Haploiden. Naturwissenschaften 57, 138–139 (1970)
Bourque, D.P., Horn, N.A., Capel, M.S.: Altered chloroplast ribosome of a streptomycin resistant mutant of Nicotiana tabacum. Plant Physiol. 59, (Suppl.) 110 (1977)
Dix, P.J., Joó, F., Maliga, P.: A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin. Mol. Gen. Genet. 157, 285–290 (1977)
Maliga, P., Lázár, G., Joó, F., H.-Nagy, A., Menczel, L.: Restoration of morphogenic potential in Nicotiana by somatic hybridisation. Mol. Gen. Genet. 157, 291–296 (1977)
Maliga, P., Lázár, G., Sváb, Z., Nagy, F.: Transient cycloheximide resistance in a tobacco cell line. Mol. Gen. Genet. 149, 267–271 (1976)
Maliga, P., Sz-Breznovits, Á., Márton, L.: Streptomycin resistant plants from callus culture of haploid tobacco. Nature (New Biol.) 244, 29–30 (1973)
Maliga, P., Sz-Breznovits, Á., Márton, L.: Non-Mendelian streptomycin-resistant tobacco mutant with altered chloroplasts and mitochondria. Nature 255, 401–402 (1975)
Melchers, G.: Einige genetische Gesichtspunkte zu sogenannten Gewebekulturen. Ber. Dtsch. bot. Ges. 78, 21–29 (1965)
Sacristán, M.D.: Karyotypic changes in callus cultures from haploid and diploid plants of crepis capillaris (L) Wallr. Chromosoma 33, 273–283 (1971)
Sacristán, M.D., Melchers, G.: The caryological analysis of plants regenerated from tumorous and other callus cultures of tobacco. Mol. Gen. Genet. 105, 317–333 (1969)
Shimada, T., Tabata, M.: Chromosome numbers in cultured pith tissues of tobacco. Jpn. J. Genet. 42, 195–201 (1967)
Sunderland, N.: Nuclear cytology. In: Plant tissue and cell culture (Street, H.E., ed.), pp. 161–190. Oxford: Blackwell 1973
Umiel, N.: The isolation of mutants in plant tissue cultures, and their use in gentic studies and plant breeding. In: Production of natural compounds by cell culture methods (Alfermann, A.W., Reinhard, E. eds.), pp. 346–352 München: Gesellschaft für Strahlen- und Umweltforschung MBH 1978
Umiel, N., Goldner, R.: Effects of streptomycin on diploid tobacco callus cultures and the isolation of resistant mutants. Protoplasma 89, 83–89 (1976)
Yurina, N.P., Oditsova, M.S., Maliga, P.: An altered chloroplast ribosomal protein in a streptomycin resistant tobacco mutant. Theor. Appl. Genet. 52, 125–128 (1978)
Author information
Authors and Affiliations
Additional information
Communicated by G. Melchers
Present address: Department of Genetics, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, U.K.
Rights and permissions
About this article
Cite this article
Maliga, P., Kiss, Z.R., Dix, P.J. et al. A streptomycin-resistant line of Nicotiana sylvestris unable to flower. Molec. Gen. Genet. 172, 13–15 (1979). https://doi.org/10.1007/BF00276209
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00276209