Skip to main content
SpringerLink
Log in

Effect of neighboring nucleotide sequences on suppression efficiency in amber mutants of T4 phage lysozyme

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Variations in suppression efficiency were observed among nonsense mutations at different locations within the lysozyme gene (e) of T4 phage. The present experiments using three amber mutants in lysozyme gene indicate such variations presumably depend upon the base sequences neighboring to the nonsense mutations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Crick, H.F.C.: Codon-anticodon pairing; The wobble hypothesis. J. molec. Biol. 19, 548 (1966)

    Google Scholar 

  • Inouye, M., Akaboshi, E., Kuroda, M., Tsugita, A.: Replacement of all tryptophan residues in T4 bacteriophage lysozyme by tyrosine residues. J. molec. Biol. 50, 71 (1970)

    Google Scholar 

  • Inouye, M., Yahata, H., Ocada, Y., Tsugita, A.: Change of tryptophan residue at the 158th position of T4 bacteriophage lysozyme into a tyrosine residue by suppression and spontaneous reversion of an amber mutant. J. molec. Biol. 33, 957 (1968)

    Google Scholar 

  • Kaplan, S., Stretton, A.O.W., Brenner, S.: Amber suppressors: efficiency of chain propagation and suppressor specific amino acid. J. molec. Biol. 14, 528 (1965)

    Google Scholar 

  • Ocada, Y., Amagase, S.,Tsugita, A.: Frameshift mutation in the lysozyme gene of bacteriophage T4: Demonstration of the insertion of five bases, and a summary of in vitro codons and lysozyme activities. J. molec. Biol. 54, 219 (1970)

    Google Scholar 

  • Tsugita, A.: Genetic code and suppression efficiency. Proc. XIIth int. Congr. Genetics, Vol. II, p. 35 (1968)

    Google Scholar 

  • Tsugita, A., Inouye, M.: Complete primary structure of T4 phage lysozyme. J. molec. Biol. 37, 201 (1968)

    Google Scholar 

  • Tsugita, A., Inouye, M., Terzaghi, E., Streisinger, G.: Purification of bacteriophage T4 lysozyme. J. biol. Chem. 243, 391 (1968)

    Google Scholar 

  • Weigert, M.G., gallucci, E., Lanka, E., Garen, A.: Characteristics of genetic code in vivo. Cold Spr. Harb. Symp. quant. Biol. 31, 145 (1966)

    Google Scholar 

  • Yahata, H., Ocada, Y., Tsugita, A.: Adjacent effect on suppression efficiency. II. study on ochre and amber mutants of T4 phage lysozyme. Molec. gen. Genet. 106, 208 (1970)

    Google Scholar 

Download references

Author information

Author notes

  1. Masayori Inouye

    Present address: Dept. Biochem. State Univ. New York at Stony Brook, New York, U.S.A.

  2. Akira Tsugita

    Present address: Dept. Microbiology, Biozentrum der Universität, Basel, Switzerland

Authors and Affiliations

  1. Laboratory of Molecular Genetics, Medical School, University of Osaka, Kita-ku Nakanoshima, Osaka

    Eiko Akaboshi, Masayori Inouye & Akira Tsugita

Authors
  1. Eiko Akaboshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masayori Inouye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akira Tsugita
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Ozeki and T. Yura

Part of this work is the thesis work of one of the authors (E.A.) and was reported at the Annual Meeting (1968) of Genetic Society of Japan

Rights and permissions

Reprints and permissions

About this article

Cite this article

Akaboshi, E., Inouye, M. & Tsugita, A. Effect of neighboring nucleotide sequences on suppression efficiency in amber mutants of T4 phage lysozyme. Molec. Gen. Genet. 149, 1–4 (1976). https://doi.org/10.1007/BF00275955

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00275955

Keywords

Search

Navigation